酿酒用糯高粱根内生溶磷菌的筛选及其促生性能研究

doi:10.19586/j.2095-2341.2024.0075

生物技术进展 ›› 2024, Vol. 14 ›› Issue (5): 832-838.DOI: 10.19586/j.2095-2341.2024.0075

• 研究论文 • 上一篇

酿酒用糯高粱根内生溶磷菌的筛选及其促生性能研究

王新叶(), 李雷, 胡亚华, 何欢, 李红霞, 李琼, 赵亮(), 章刚容, 潘琪, 汤瑄, 张埔涤, 居文静, 杨正烁

茅台学院酿酒工程系，贵州遵义 564500

收稿日期:2024-04-09 接受日期:2024-04-18 出版日期:2024-09-25 发布日期:2024-10-22
通讯作者: 赵亮
作者简介:王新叶 E-mail：wangxinye@mtxy.edu.cn；
基金资助:
遵义市科学技术局、茅台学院市校联合科技研发资金项目(遵市科合HZ字〔2020〕317号);茅台学院高层次人才科研启动项目(mygccrc〔2022〕023)

Isolation and Screening of Phosphate Solubilizing Bacteria and its Growth-promoting Effect on Glutinous Sorghum for Brewing

Xinye WANG(), Lei LI, Yahua HU, Huan HE, Hongxia LI, Qiong LI, Liang ZHAO(), Gangrong ZHANG, Qi PAN, Xuan TANG, Pudi ZHANG, Wenjing JU, Zhengshuo YANG

Department of Brewing Engineering，Moutai Institute，Guizhou Zunyi 564500，China

Received:2024-04-09 Accepted:2024-04-18 Online:2024-09-25 Published:2024-10-22
Contact: Liang ZHAO

摘要/Abstract

摘要：

酿酒用糯高粱生长过程需要大量的磷元素，缺磷会造成糯高粱的减产，但是化肥的大量施用会造成土壤肥力下降，为开发促进糯高粱生长的溶磷菌剂，使用溶磷圈法从糯高粱根内筛选具有溶磷功能的菌株，对获得的菌株进行菌种鉴定，并将该菌株制成菌悬液接种到糯高粱根部，测定糯高粱的生长指标，评估该菌株对糯高粱的促生长性能。从糯高粱根中筛选得到了6株溶磷菌，其中溶磷圈直径与菌落直径比值最大的是菌株GP4-1，为2.2。基于菌株16S rDNA序列的系统发育分析，GP4-1属于伯克霍尔德菌(Burkholderia sp.)。将GP4-1菌悬液接种到高粱幼苗根部30 d后，高粱幼苗的株高增加了4.46%，鲜重增加了19.69%，干重增加了100%，植株全磷含量增加了2.65%。糯高粱根内生菌GP4-1通过促进糯高粱植株对磷元素的吸收促进高粱的生长，为针对糯高粱的溶磷菌剂的开发和应用奠定了基础。

关键词: 糯高粱, 溶磷菌, 促植物生长

Abstract:

The growth process of glutinous sorghum for brewing requires a large amount of phosphorus element， and the lack of phosphorus would result in the yield reduction， but the long time of fertilizer use would cause the decline of soil fertility. In order to develop the phosphorus soluble bacteria agent to promote glutinous sorghum growth， this study used the phosphorus soluble ring method to screen the strains with phosphorus soluble function from glutinous sorghum roots， and the strains obtained were identified. The suspension was inoculated into the root of glutinous sorghum seedlings， the glutinous sorghum growth traits were determined， and the growth promotion performance of the strain on glutinous sorghum was evaluated. In this study， six strains of phosphorus solubilizing bacteria were selected from glutinous sorghum roots， isolate GP4-1 had the largest ratio of phosphorus solubilizing circle diameter to colony diameter， which was 2.2. Based on phylogenetic analysis of 16S rDNA sequences， GP4-1 belonged to Burkholderia sp. GP4-1. After 30 days of inoculation with GP4-1 bacterial suspension， plant height， fresh weight， dry weight and the total phosphorus content of sorghum seedlings increased by 4.46%， 19.69%， 100% and 2.65%， respectively. The root endophyte GP4-1 promoted glutinous sorghum growth by promoting phosphorus absorption. This study laid a foundation for the development and application of phosphorus solubilizing bacteria for glutinous sorghum.

Key words: glutinous sorghum, phosphate-solubilizing bacteria, plant growth promoting

中图分类号:

S514

王新叶, 李雷, 胡亚华, 何欢, 李红霞, 李琼, 赵亮, 章刚容, 潘琪, 汤瑄, 张埔涤, 居文静, 杨正烁. 酿酒用糯高粱根内生溶磷菌的筛选及其促生性能研究[J]. 生物技术进展, 2024, 14(5): 832-838.

Xinye WANG, Lei LI, Yahua HU, Huan HE, Hongxia LI, Qiong LI, Liang ZHAO, Gangrong ZHANG, Qi PAN, Xuan TANG, Pudi ZHANG, Wenjing JU, Zhengshuo YANG. Isolation and Screening of Phosphate Solubilizing Bacteria and its Growth-promoting Effect on Glutinous Sorghum for Brewing[J]. Current Biotechnology, 2024, 14(5): 832-838.

图/表 5

表1 菌株的溶磷能力测定

Table 1 Determination of phosphorus solubilization ability of isolated strains

菌株编号	溶磷圈直径（D）/cm			菌落直径（d）/cm			D/d
菌株编号	①	②	③	①	②	③	①	②	③	平均值±标准差
GP4-1	1.4	1.1	1.0	0.6	0.5	0.5	2.3	2.2	2.0	2.20±0.15
GP4-2	2.1	2.0	2.0	1.6	1.6	1.5	1.3	1.3	1.3	1.30±0
GP4-3	2.2	2.1	2.3	2.0	1.9	1.9	1.1	1.1	1.2	1.10±0.06
GP4-4	2.3	2.1	2.0	1.9	1.8	1.5	1.2	1.2	1.3	1.20±0.06
GP4-5	2.5	2.6	2.0	2.0	2.1	1.6	1.3	1.2	1.3	1.20±0.06
GP4-6	1.8	2.0	1.9	0.8	1.1	1.0	2.3	1.8	1.9	2.00±0.26

图1 菌株的菌落形态及溶磷圈

Fig. 1 Colony morphology and phosphorus-soluble circle of the strain

图2 基于16S rDNA序列的系统发育树

Fig. 2 Phylogenetic tree based on 16S rDNA sequence

图3 糯高粱种子萌发指标的测定A：糯高粱种子发芽势；B：糯高粱种子发芽率

Fig. 3 Determination of sorghum seed germination index

图4 糯高粱生长指标统计分析A：株高；B：鲜重；C：干重；D：全磷含量。不同小写字母表示处理间差异具有统计学意义（P<0.05）。

Fig. 4 Determination of sorghum seed germination index

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酿酒用糯高粱根内生溶磷菌的筛选及其促生性能研究

Isolation and Screening of Phosphate Solubilizing Bacteria and its Growth-promoting Effect on Glutinous Sorghum for Brewing

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