冷等离子体处理对盐胁迫下燕麦种子萌发及幼苗生长的影响

doi:10.19586/j.2095-2341.2023.0167

生物技术进展 ›› 2024, Vol. 14 ›› Issue (3): 413-421.DOI: 10.19586/j.2095-2341.2023.0167

• 研究论文 • 上一篇

冷等离子体处理对盐胁迫下燕麦种子萌发及幼苗生长的影响

张奇雨(), 吕晓桂, 包锦()

内蒙古农业大学理学院，呼和浩特 010018

收稿日期:2023-12-20 接受日期:2024-03-25 出版日期:2024-05-25 发布日期:2024-06-18
通讯作者: 包锦
作者简介:张奇雨 E-mail: 2962888599@qq.com；
基金资助:
内蒙古自治区高等学校科学研究项目(NJZZ23036);内蒙古自治区直属高校基本科研业务费项目(BR230111)

Effect of Cold Plasma Treatment on Seed Germination and Seedling Growth of Oats Under Salt Stress

Qiyu ZHANG(), Xiaogui LYU, Jin BAO()

College of Science，Inner Mongolia Agricultural University，Hohhot 010018，China

Received:2023-12-20 Accepted:2024-03-25 Online:2024-05-25 Published:2024-06-18
Contact: Jin BAO

摘要/Abstract

摘要：

为探究冷等离子体处理对盐胁迫下燕麦耐盐性的影响，以坝莜14号燕麦种子为试验材料，选用5和6 kV的电压对燕麦种子进行不同时长（30 s、15 s×2）的冷等离子体处理，测定不同浓度的NaCl 溶液(0.5、1.0、1.5 g·L^-1)胁迫下燕麦种子萌发和幼苗生长及生理指标。结果发现，冷等离子体可以显著提高燕麦种皮亲水性及燕麦种子的吸水率，缓解盐胁迫对燕麦株高和根长的影响。基于隶属函数对燕麦抗盐能力进行综合评价分析发现，冷等离子体处理参数为5 kV、30 s和6 kV、15 s×2时，1.5 g·L^-1浓度胁迫下燕麦的抗盐能力提高，且随着胁迫强度的增加，5 kV、30 s参数下，冷等离子体处理对盐胁迫的缓解作用效果逐渐增强。

关键词: 燕麦, 盐胁迫, 冷等离子体, 种子萌发, 幼苗生长

Abstract:

To investigate the effect of cold plasma treatment on salt tolerance of oats under salt stress， the oat seeds of Bayou 14 were used as experimental materials， and different durations （30 s， 15 s×2） of 5 and 6 kV were applied to the oat seeds at different times cold plasma treatment was used to determine the germination of oat seeds， seedling growth， and physiological indicators under different concentrations of NaCl solution （0.5， 1.0， 1.5 g·L^-1） stress. The results showed that cold plasma can significantly improve the hydrophilicity of oat seed coat and the water absorption rate of oat seeds， and could alleviate the effects of salt stress on oat plant height and root length. Based on the comprehensive evaluation and analysis of oat salt resistance using membership functions， it was found that the cold plasma treatment parameters were 5 kV， 30 s， and 6 kV， 15 s×2， respectively， the salt resistance of oats under stress concentration of 1.5 g·L^-1 was increased. Moreover， as the stress intensity increases， the alleviating effect of cold plasma treatment on salt stress gradually increases under 5 kV and 30 s parameters.

Key words: oat, salt stress, cold plasma, seed germination, seedling growth

中图分类号:

S512.6

张奇雨, 吕晓桂, 包锦. 冷等离子体处理对盐胁迫下燕麦种子萌发及幼苗生长的影响[J]. 生物技术进展, 2024, 14(3): 413-421.

Qiyu ZHANG, Xiaogui LYU, Jin BAO. Effect of Cold Plasma Treatment on Seed Germination and Seedling Growth of Oats Under Salt Stress[J]. Current Biotechnology, 2024, 14(3): 413-421.

图/表 9

图1 等离子体发生装置注：1—铝箔；2—等离子体射流；3—燕麦种子；4—传送带；5—低压电极；6—低温等离子体电源；7—示波器；8—扁口石英管；9—高压探头。

Fig.1 Plasma generation device

表1 各组燕麦种子处理参数

Table 1 Treatment parameters for each group of oat seeds

组别	CK1 CK2 CK3	A1 A2 A3	B1 B2 B3	C1 C2 C3
电压/kV	-	5	6	6
处理时间/s	-	30 30 30	15 15 15	30 30 30
处理次数	-	1 1 1	2 2 2	1 1 1
溶液浓度 /(g·L^-1)	0.5 1.0 1.5	0.5 1.0 1.5	0.5 1.0 1.5	0.5 1.0 1.5

图2 冷等离子体处理对燕麦种子吸水率和亲水性的影响注：不同小写字母表示不同处理间在P<0.05水平上具有统计学意义。

Fig. 2 Effects of cold plasma treatment on water absorption and hydrophilicity of oat seeds

图3 冷等离子体处理对NaCl胁迫下燕麦种子发芽势和发芽率的影响注：不同小写字母表示不同处理间在P<0.05水平上具有统计学意义。

Fig. 3 Effects of cold plasma treatment on germination potential and germination rate of oat seeds under NaCl stress

图4 冷等离子体处理对NaCl胁迫下燕麦株高和根长的影响注：不同小写字母表示不同处理间在P<0.05水平上具有统计学意义。

Fig. 4 Effects of cold plasma treatment on plant length and root length of oats under NaCl stress

图5 冷等离子体处理对NaCl胁迫下燕麦幼苗叶绿素含量的影响注：不同小写字母表示不同处理间在P<0.05水平上具有统计学意义。

Fig. 5 Effects of cold plasma treatment on chlorophyll content of oat seedlings under NaCl stress

图6 冷等离子体处理对NaCl胁迫下燕麦幼苗丙二醛含量的影响注：不同小写字母表示不同处理间在P<0.05水平上具有统计学意义。

Fig. 6 Effects of cold plasma treatment on malondialdehyde content of oat seedlings under NaCl stress

表2 各指标相关性分析

Table 2 Correlation analysis of various indicators

指标	表面接触角	吸水率	发芽势	发芽率	株高	根长	叶绿素	丙二醛
表面接触角	1.000
吸水率	-0.877**	1.000
发芽势	0.411	-0.296	1.000
发芽率	0.626*	-0.532*	0.922**	1.000
株高	0.139	0.066	0.909**	0.741**	1.000
根长	0.006	0.183	0.761**	0.597*	0.915**	1.000
叶绿素	0.410	-0.404	0.577*	0.590*	0.580*	0.597*	1.000
丙二醛	-0.479	0.299	-0.318	-0.471	-0.327	-0.360	-0.503*	1.000

表3 冷等离子体处理下燕麦抗盐性的综合评价结果

Table 3 Comprehensive evaluation results of salt resistance of oats under cold plasma treatment

组别	综合指标X₁	综合指标X₂	综合指标X₃	隶属函数值μ₁	隶属函数值μ₂	隶属函数值μ₃	综合评价F
CK1	1.791 2	-0.539 7	1.313 6	1.000 0	0.377 6	0.931 1	0.820 2
CK2	1.160 5	-1.061 0	-0.502 5	0.820 2	0.237 4	0.326 5	0.605 9
CK3	-0.146 7	-1.944 2	0.082 0	0.447 6	0.000 0	0.521 1	0.331 4
A1	0.174 7	0.665 8	1.520 4	0.539 2	0.701 6	1.000 0	0.633 5
A2	0.370 6	0.427 0	-1.483 0	0.595 1	0.637 4	0.000 0	0.543 1
A3	-0.222 0	-0.250 2	-1.434 1	0.426 1	0.455 4	0.016 3	0.390 4
B1	0.729 1	1.775 6	0.323 9	0.697 2	1.000 0	0.601 6	0.770 9
B2	-0.655 9	0.787 3	-0.147 4	0.302 4	0.734 3	0.444 7	0.437 3
B3	-1.190 8	0.708 4	-0.066 9	0.150 0	0.713 1	0.471 5	0.340 4
C1	0.499 5	0.507 1	-0.491 9	0.631 8	0.659 0	0.330 0	0.607 0
C2	-0.793 4	-0.490 0	-0.474 3	0.263 2	0.390 9	0.335 9	0.306 4
C3	-1.716 8	-0.586 0	1.360 1	0.000 0	0.365 1	0.946 6	0.202 5

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冷等离子体处理对盐胁迫下燕麦种子萌发及幼苗生长的影响

Effect of Cold Plasma Treatment on Seed Germination and Seedling Growth of Oats Under Salt Stress

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