3种植物对复合重金属的胁迫效应及富集转运特征

doi:10.19586/j.2095-2341.2022.0182

生物技术进展 ›› 2023, Vol. 13 ›› Issue (3): 425-434.DOI: 10.19586/j.2095-2341.2022.0182

3种植物对复合重金属的胁迫效应及富集转运特征

刘潇冉(), 张玉蕾, 武宇鹏()

太原科技大学环境与资源学院，太原 030024

收稿日期:2022-11-07 接受日期:2023-01-06 出版日期:2023-05-25 发布日期:2023-06-12
通讯作者: 武宇鹏
作者简介:刘潇冉 E-mail: 1252786866@qq.com；
基金资助:
山西省重点研发计划项目(201903D321058)

Stress Effects， Enrichment and Transport Characteristics of Three Plants on Complex Heavy Metals

Xiaoran LIU(), Yulei ZHANG, Yupeng WU()

College of Environment and Resources，Taiyuan University of Science and Technology，Taiyuan 030024，China

Received:2022-11-07 Accepted:2023-01-06 Online:2023-05-25 Published:2023-06-12
Contact: Yupeng WU

摘要/Abstract

摘要：

为了探讨紫花苜蓿、黑麦草和玉米这3种植物在不同种植模式下对土壤中重金属的富集效果和受重金属毒害的胁迫效应。通过盆栽土培实验，模拟5个不同浓度复合重金属（Zn、Cd、Cr）的污染条件，在单作、间作和间作添加微生物菌剂3种模式下种植3种植物，待45 d收获后测定植物的株高、根长、鲜重、植物叶绿素、根际土壤和植物体内重金属含量等生理指标。结果表明，苜蓿在3种种植模式下生理指标呈“低浓度促进，高浓度抑制”现象，玉米和黑麦草变化趋势不明显，苜蓿在单作模式下长势优于其他2种模式，玉米和黑麦草则反之。在间作模式中，低浓度重金属条件下土壤pH下降明显。3种植物地上和地下部重金属含量随着土壤重金属浓度的升高而增加，3种植物在间作加菌种植模式下对土壤重金属的富集和转运系数均比其他2种模式高。其中，苜蓿在单作种植模式下对Zn的转运系数为1.25，而在间作加菌模式下高达2.37。黑麦草和玉米在间作加菌种植模式下对Cd的富集系数均大于1。黑麦草在间作加菌种植模式下，对Cr的富集系数高达2.29，转运系数为0.87。3种植物在间作加菌种植模式下，对不同浓度梯度重金属的耐受性明显好于其他种植模式。黑麦草在间作加菌种植模式下的生理指标较其他2种模式效果好，在低浓度的重金属胁迫下显著降低了土壤pH，提高了植物叶绿素含量。玉米在间作模式下对高浓度Cd的富集系数大于1。总之，3种植物在间作加菌模式下对重金属的富集效果最好，建议在尾矿修复中推广应用。

关键词: 种植模式, 重金属, 胁迫, 富集

Abstract:

The aim of this study was to investigate the enrichment effects of heavy metals in soil and the stress effects of heavy metals poisoning on three plants， alfalfa， ryegrass and maize， under different planting patterns. Through potted soil culture experiment， simulating five different concentrations of heavy metals （Zn， Cd， Cr） pollution condition， the three plants were planted under three modes of monocropping， intercropping and interplanting with microbial inoculants. After 45 days of harvest， physiological indexes including plant height， root length， fresh weight， plant chlorophyll， rhizosphere and heavy metal content in plant were measured. The results showed that the physiological indexes of alfalfa under the three planting patterns were promoted by low concentration and inhibited by high concentration. The change trend of corn and ryegrass was not obvious. Alfalfa under the mono-cropping pattern was better than the other two patterns， while corn and ryegrass were vice versa. In the intercropping mode， the pH of soil decreased obviously under the condition of low concentration of heavy metals. The concentrations of heavy metals in the above-ground and underground parts of the three plants increased with the increase of soil heavy metal concentrations. The enrichment and transport coefficients of heavy metals in the soil of the three plants under intercropping with fungi were higher than those in the other two modes. The transfer coefficient of Zn to alfalfa under monocropping was 1.25， while the transfer coefficient of Zn to alfalfa under intercropping was 2.37. The enrichment coefficient of Cd in ryegrass and corn under intercropping with fungi was greater than 1. The enrichment coefficient and transport coefficient of Cr for ryegrass were as high as 2.29 and 0.87， respectively. The tolerance of the three plants to heavy metals of different concentration gradients was significantly better than that of other planting modes under intercropping and adding bacteria. The physiological indexes of ryegrass under intercropping and adding fungi were better than those of the other two modes， and the pH of soil was significantly reduced and the plant chlorophyll content was increased under the stress of low concentration of heavy metals. The enrichment coefficient of Cd was greater than 1 under intercropping mode. In conclusion， the three plants had the best enrichment effect on heavy metals under the intercropping and adding bacteria mode， and it was suggested to be popularized and applied in tailings remediation.

Key words: planting pattern, heavy metals, stress, enrichment

中图分类号:

Q945.78

刘潇冉, 张玉蕾, 武宇鹏. 3种植物对复合重金属的胁迫效应及富集转运特征[J]. 生物技术进展, 2023, 13(3): 425-434.

Xiaoran LIU, Yulei ZHANG, Yupeng WU. Stress Effects， Enrichment and Transport Characteristics of Three Plants on Complex Heavy Metals[J]. Current Biotechnology, 2023, 13(3): 425-434.

图/表 10

表1 铁尾矿污染土壤营养成分指标检测结果

Table 1 Test results of nutrient composition index of soil polluted by iron tailings

营养成分	污染土壤	浓度适宜范围
有机质	8.06 g·kg^-1	15 g·kg^-1
碱解氮	12.0 mg·kg^-1	100 mg·kg^-1
速效磷	9.4 mg·kg^-1	100 mg·kg^-1
速效钾	55 mg·kg^-1	160 mg·kg^-1
全氮	1 047 mg·kg^-1	1 300 mg·kg^-1
全钾	0.60 mg·kg^-1	16.06 g·kg^-1

表2 土壤中重金属模拟浓度设置

Table 2 Simulated concentration settings of heavy metals in soil

重金属	土壤质量标准/（mg·kg^-1）	浓度梯度设置/（mg·kg^-1）
重金属	土壤质量标准/（mg·kg^-1）	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ
Cd	0.8	0	10	20	30	40
Cr	250	0	100	200	400	600
Zn	300	0	100	200	400	600

表3 3种植物在3种种植模式下生物量的变化

Table 3 Biomass changes of the three plants under the three planting modes

浓度梯度		植物株高/cm			根长/cm			地上部鲜重/(g·盆^-1)			地下部鲜重/(g·盆^-1)			总生物量/(g·盆^-1)			植物干重/(g·盆^-1)
浓度梯度		苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草		玉米
种植模式	Ⅰ	$10.73 ± 0.61 a$	$29.33 ± 4.08 a b$	$36.30 ± 4.49 a$	$10.80 ± 0.33 a$	$9.80 ± 1.81 a$	$14.53 ± 1.76 b$	$1.23 ± 0.16 c$	$4.17 ± 0.58 b$	$2.67 ± 0.26 a$	$0.38 ± 0.03 b c$	$0.26 ± 0.04 d$	$1.26 ± 0.14 d$	$1.60 ± 0.14 d$	$4.43 ± 0.60 b$	$3.93 ± 0.40 b$	$0.28 ± 0.02 c$		$0.55 ± 0.08 b$	$0.55 ± 0.06 b c$
	Ⅱ	$11.33 ± 1.74 a$	$25.40 ± 2.30 b$	$33.83 ± 1.19 a$	$12.10 ± 1.56 a$	$10.40 ± 0.83 a$	$18.93 ± 0.56 b$	$1.50 ± 0.38 c$	$3.67 ± 0.38 b$	$2.32 ± 0.19 a b$	$0.46 ± 0.02 b$	$0.93 ± 0.08 c$	$3.06 ± 0.15 a$	$1.9 ± 0.40 c d$	$4.59 ± 0.45 b$	$5.38 ± 0.06 a$	$0.48 ± 0.10 b$		$0.57 ± 0.05 b$	$0.73 ± 0.03 a$
	Ⅲ	$11.07 ± 0.83 a$	$27.20 ± 0.91 a b$	$30.80 ± 2.22 a$	$12.27 ± 1.53 a$	$11.19 ± 0.29 a$	$18.83 ± 1.28 a$	$4.70 ± 0.11 a$	$4.00 ± 0.09 b$	$1.97 ± 0.25 b$	$0.71 ± 0.07 a$	$1.11 ± 0.03 b c$	$2.19 ± 0.15 b$	$5.41 ± 0.18 a$	$5.12 ± 0.10 b$	$4.16 ± 0.39 b$	$1.00 ± 0.05 a$		$0.63 ± 0.02 b$	$0.59 ± 0.05 b$
	Ⅳ	$7.10 ± 0.88 a$	$32.20 ± 2.37 a$	$33.13 ± 1.30 a$	$7.43 ± 1.06 b$	$12.45 ± 0.92 a$	$14.30 ± 0.59 b$	$2.33 ± 0.27 b$	$6.22 ± 0.62 a$	$2.61 ± 0.13 a$	$0.27 ± 0.03 c d$	$2.46 ± 0.28 a$	$1.74 ± 0.10 c$	$2.6 ± 0.30 b c$	$8.67 ± 0.90 a$	$4.34 ± 0.23 b$	$0.49 ± 0.03 b$		$1.13 ± 0.14 a$	$0.64 ± 0.03 a b$
	Ⅴ	$5.03 ± 1.10 b$	$28.53 ± 1.04 a b$	$19.83 ± 2.71 b$	$5.70 ± 1.04 b$	$11.21 ± 1.17 a$	$12.87 ± 1.76 b$	$2.71 ± 0.18 b$	$3.91 ± 0.33 b$	$1.43 ± 0.18 c$	$0.22 ± 0.05 d$	$1.29 ± 0.11 b$	$1.52 ± 0.21 c d$	$2.93 ± 0.23 b$	$5.20 ± 0.43 b$	$2.95 ± 0.38 c$	$0.60 ± 0.03 b$		$0.68 ± 0.05 b$	$0.45 ± 0.06 c$
间作	Ⅰ	$8.27 ± 0.90 a$	$31.60 ± 0.22 a$	$46.00 ± 3.32 a$	$8.47 ± 1.17 a$	$12.29 ± 0.44 a b$	$15.90 ± 0.78 a$	$0.94 ± 0.31 a$	$4.99 ± 0.08 b$	$5.82 ± 0.48 b c$	$0.31 ± 0.06 a$	$0.93 ± 0.01 c$	$2.20 ± 0.09 c$	$1.25 ± 0.36 a$	$5.92 ± 0.08 c$	$8.02 ± 0.57 b$	$0.32 ± 0.09 a$		$0.74 ± 0.04 b c$	$1.07 ± 0.07 b c$
	Ⅱ	$9.93 ± 2.12 a$	$33.97 ± 3.26 a$	$43.17 ± 1.72 a$	$10.53 ± 2.39 a$	$13.86 ± 1.47 a$	$18.63 ± 0.78 a$	$0.99 ± 0.34 a$	$5.33 ± 0.54 b$	$3.76 ± 0.31 c$	$0.32 ± 0.06 a$	$0.86 ± 0.08 c$	$3.70 ± 0.16 b$	$1.31 ± 0.40 a$	$6.19 ± 0.62 b c$	$7.47 ± 0.47 b$	$0.29 ± 0.08 a$		$0.75 ± 0.08 b c$	$1.02 ± 0.06 c$
	Ⅲ	$8.13 ± 1.14 a b$	$32.60 ± 0.78 a$	$44.20 ± 7.25 a$	$8.50 ± 1.71 a$	$11.58 ± 0.28 a b$	$16.87 ± 2.32 a$	$0.20 ± 0.11 b$	$5.04 ± 0.22 b$	$6.22 ± 1.03 b$	$0.29 ± 0.05 a$	$2.84 ± 0.02 b$	$2.27 ± 0.29 c$	$0.49 ± 0.15 b$	$7.88 ± 0.22 b$	$8.49 ± 1.32 b$	$0.17 ± 0.01 b$		$0.95 ± 0.03 b$	$1.18 ± 0.18 b$
	Ⅳ	$7.17 ± 0.40 a b$	$25.37 ± 8.66 a$	$43.83 ± 3.66 a$	$7.60 ± 0.57 a$	$10.37 ± 2.59 a b$	$18.33 ± 1.56 a$	$0.12 ± 0.06 b$	$3.95 ± 1.36 b$	$17.96 ± 1.53 a$	$0.28 ± 0.02 a$	$0.99 ± 0.26 c$	$6.90 ± 0.58 a$	$0.41 ± 0.07 b$	$4.94 ± 1.62 c$	$24.86 ± 2.09 a$	$0.10 ± 0.01 a b$		$0.62 ± 0.20 c$	$3.08 ± 0.22 a$
	Ⅴ	$5.83 ± 0.62 b$	$27.43 ± 1.02 a$	$37.70 ± 3.27 a$	$7.87 ± 0.21 a$	$10.16 ± 0.36 b$	$16.10 ± 1.42 a$	$1.30 ± 0.03 a$	$7.75 ± 0.20 a$	$7.02 ± 0.61 b$	$0.29 ± 0.01 a$	$3.57 ± 0.16 a$	$2.60 ± 0.13 c$	$1.59 ± 0.04$ a	$11.32 ± 0.32 a$	$9.62 ± 0.75 b$	$0.29 ± 0.01 a$		$1.46 ± 0.03 a$	$1.38 ± 0.08 b$
间作加菌	Ⅰ	$6.27 ± 1.94 a$	$31.77 ± 1.70 a$	$43.47 ± 5.29 a$	$6.50 ± 2.16 a$	$12.60 ± 0.98 a$	$16.10 ± 2.01 a$	$0.08 ± 0.05 b$	$7.77 ± 0.46 b c$	$4.24 ± 0.55 c$	$0.16 ± 0.04 b$	$1.99 ± 0.13 d$	$1.65 ± 0.21 b$	$0.24 ± 0.09 b$	$9.76 ± 0.57 c$	$5.89 ± 0.76 b$	$0.11 ± 0.03 b$		$1.07 ± 0.01 c$	$0.79 ± 0.10 b$
	Ⅱ	$9.23 ± 2.04 a$	$28.57 ± 3.90 a$	$36.70 ± 3.60 a b$	$9.50 ± 2.73 a$	$11.48 ± 1.40 a b$	$12.13 ± 1.15 b$	$0.19 ± 0.08 b$	$6.60 ± 1.01 c$	$6.13 ± 0.59 b$	$0.35 ± 0.07 a$	$0.73 ± 0.09 e$	$3.83 ± 0.35 a$	$0.50 ± 0.15 a b$	$7.33 ± 1.08 d$	$9.96 ± 0.94 a$	$0.24 ± 0.05 a$		$0.93 ± 0.09 c$	$1.39 ± 0.13 a$
	Ⅲ	$7.90 ± 1.99 a$	$33.43 ± 0.84 a$	$32.73 ± 3.84 a b$	$8.57 ± 1.97 a$	$12.02 ± 0.65 a$	$11.80 ± 1.27 b$	$0.42 ± 0.25 a b$	$8.76 ± 0.19 b$	$4.35 ± 0.73 c$	$0.28 ± 0.05 a b$	$4.89 ± 0.54 a$	$2.02 ± 0.24 b$	$0.70 ± 0.29 a$	$13.65 ± 0.72 b$	$6.36 ± 0.97 b$	$0.18 ± 0.07 a b$		$1.61 ± 0.13 b$	$0.85 ± 0.17 b$
	Ⅳ	$6.10 ± 2.51 a$	$30.60 ± 0.45 a$	$40.10 ± 0.45 b$	$6.47 ± 2.04 a$	$11.75 ± 0.21 a b$	$16.07 ± 0.17 a$	$0.54 ± 0.15 a$	$6.47 ± 0.07 c$	$7.88 ± 0.03 a$	$0.25 ± 0.05 a b$	$2.89 ± 0.12 c$	$2.09 ± 0.05 b$	$0.79 ± 0.20 a$	$9.36 ± 0.14 c$	$9.97 ± 0.08 a$	$0.19 ± 0.05 a b$		$1.15 ± 0.03 c$	$1.42 ± 0.01 a$
	Ⅴ	$5.17 ± 0.46 a$	$28.33 ± 2.92 a$	$43.73 ± 2.46 a$	$6.17 ± 0.49 a$	$9.58 ± 0.97 b$	$18.23 ± 1.01 a$	$0.34 ± 0.03 a b$	$11.87 ± 0.50 a$	$5.12 ± 0.32 b c$	$0.24 ± 0.01 a b$	$3.83 ± 0.31 b$	$3.44 ± 0.18 a$	$0.5 ± 0.04 a b$	$15.70 ± 0.80 a$	$8.56 ± 0.50 a$	$0.17 ± 0.01 a b$		$1.98 ± 0.12 a$	$1.24 ± 0.07 a$

表3 3种植物在3种种植模式下生物量的变化

Table 3 Biomass changes of the three plants under the three planting modes

浓度梯度		植物株高/cm			根长/cm			地上部鲜重/(g·盆^-1)			地下部鲜重/(g·盆^-1)			总生物量/(g·盆^-1)			植物干重/(g·盆^-1)
浓度梯度		苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草		玉米
种植模式	Ⅰ	$10.73 ± 0.61 a$	$29.33 ± 4.08 a b$	$36.30 ± 4.49 a$	$10.80 ± 0.33 a$	$9.80 ± 1.81 a$	$14.53 ± 1.76 b$	$1.23 ± 0.16 c$	$4.17 ± 0.58 b$	$2.67 ± 0.26 a$	$0.38 ± 0.03 b c$	$0.26 ± 0.04 d$	$1.26 ± 0.14 d$	$1.60 ± 0.14 d$	$4.43 ± 0.60 b$	$3.93 ± 0.40 b$	$0.28 ± 0.02 c$		$0.55 ± 0.08 b$	$0.55 ± 0.06 b c$
	Ⅱ	$11.33 ± 1.74 a$	$25.40 ± 2.30 b$	$33.83 ± 1.19 a$	$12.10 ± 1.56 a$	$10.40 ± 0.83 a$	$18.93 ± 0.56 b$	$1.50 ± 0.38 c$	$3.67 ± 0.38 b$	$2.32 ± 0.19 a b$	$0.46 ± 0.02 b$	$0.93 ± 0.08 c$	$3.06 ± 0.15 a$	$1.9 ± 0.40 c d$	$4.59 ± 0.45 b$	$5.38 ± 0.06 a$	$0.48 ± 0.10 b$		$0.57 ± 0.05 b$	$0.73 ± 0.03 a$
	Ⅲ	$11.07 ± 0.83 a$	$27.20 ± 0.91 a b$	$30.80 ± 2.22 a$	$12.27 ± 1.53 a$	$11.19 ± 0.29 a$	$18.83 ± 1.28 a$	$4.70 ± 0.11 a$	$4.00 ± 0.09 b$	$1.97 ± 0.25 b$	$0.71 ± 0.07 a$	$1.11 ± 0.03 b c$	$2.19 ± 0.15 b$	$5.41 ± 0.18 a$	$5.12 ± 0.10 b$	$4.16 ± 0.39 b$	$1.00 ± 0.05 a$		$0.63 ± 0.02 b$	$0.59 ± 0.05 b$
	Ⅳ	$7.10 ± 0.88 a$	$32.20 ± 2.37 a$	$33.13 ± 1.30 a$	$7.43 ± 1.06 b$	$12.45 ± 0.92 a$	$14.30 ± 0.59 b$	$2.33 ± 0.27 b$	$6.22 ± 0.62 a$	$2.61 ± 0.13 a$	$0.27 ± 0.03 c d$	$2.46 ± 0.28 a$	$1.74 ± 0.10 c$	$2.6 ± 0.30 b c$	$8.67 ± 0.90 a$	$4.34 ± 0.23 b$	$0.49 ± 0.03 b$		$1.13 ± 0.14 a$	$0.64 ± 0.03 a b$
	Ⅴ	$5.03 ± 1.10 b$	$28.53 ± 1.04 a b$	$19.83 ± 2.71 b$	$5.70 ± 1.04 b$	$11.21 ± 1.17 a$	$12.87 ± 1.76 b$	$2.71 ± 0.18 b$	$3.91 ± 0.33 b$	$1.43 ± 0.18 c$	$0.22 ± 0.05 d$	$1.29 ± 0.11 b$	$1.52 ± 0.21 c d$	$2.93 ± 0.23 b$	$5.20 ± 0.43 b$	$2.95 ± 0.38 c$	$0.60 ± 0.03 b$		$0.68 ± 0.05 b$	$0.45 ± 0.06 c$
间作	Ⅰ	$8.27 ± 0.90 a$	$31.60 ± 0.22 a$	$46.00 ± 3.32 a$	$8.47 ± 1.17 a$	$12.29 ± 0.44 a b$	$15.90 ± 0.78 a$	$0.94 ± 0.31 a$	$4.99 ± 0.08 b$	$5.82 ± 0.48 b c$	$0.31 ± 0.06 a$	$0.93 ± 0.01 c$	$2.20 ± 0.09 c$	$1.25 ± 0.36 a$	$5.92 ± 0.08 c$	$8.02 ± 0.57 b$	$0.32 ± 0.09 a$		$0.74 ± 0.04 b c$	$1.07 ± 0.07 b c$
	Ⅱ	$9.93 ± 2.12 a$	$33.97 ± 3.26 a$	$43.17 ± 1.72 a$	$10.53 ± 2.39 a$	$13.86 ± 1.47 a$	$18.63 ± 0.78 a$	$0.99 ± 0.34 a$	$5.33 ± 0.54 b$	$3.76 ± 0.31 c$	$0.32 ± 0.06 a$	$0.86 ± 0.08 c$	$3.70 ± 0.16 b$	$1.31 ± 0.40 a$	$6.19 ± 0.62 b c$	$7.47 ± 0.47 b$	$0.29 ± 0.08 a$		$0.75 ± 0.08 b c$	$1.02 ± 0.06 c$
	Ⅲ	$8.13 ± 1.14 a b$	$32.60 ± 0.78 a$	$44.20 ± 7.25 a$	$8.50 ± 1.71 a$	$11.58 ± 0.28 a b$	$16.87 ± 2.32 a$	$0.20 ± 0.11 b$	$5.04 ± 0.22 b$	$6.22 ± 1.03 b$	$0.29 ± 0.05 a$	$2.84 ± 0.02 b$	$2.27 ± 0.29 c$	$0.49 ± 0.15 b$	$7.88 ± 0.22 b$	$8.49 ± 1.32 b$	$0.17 ± 0.01 b$		$0.95 ± 0.03 b$	$1.18 ± 0.18 b$
	Ⅳ	$7.17 ± 0.40 a b$	$25.37 ± 8.66 a$	$43.83 ± 3.66 a$	$7.60 ± 0.57 a$	$10.37 ± 2.59 a b$	$18.33 ± 1.56 a$	$0.12 ± 0.06 b$	$3.95 ± 1.36 b$	$17.96 ± 1.53 a$	$0.28 ± 0.02 a$	$0.99 ± 0.26 c$	$6.90 ± 0.58 a$	$0.41 ± 0.07 b$	$4.94 ± 1.62 c$	$24.86 ± 2.09 a$	$0.10 ± 0.01 a b$		$0.62 ± 0.20 c$	$3.08 ± 0.22 a$
	Ⅴ	$5.83 ± 0.62 b$	$27.43 ± 1.02 a$	$37.70 ± 3.27 a$	$7.87 ± 0.21 a$	$10.16 ± 0.36 b$	$16.10 ± 1.42 a$	$1.30 ± 0.03 a$	$7.75 ± 0.20 a$	$7.02 ± 0.61 b$	$0.29 ± 0.01 a$	$3.57 ± 0.16 a$	$2.60 ± 0.13 c$	$1.59 ± 0.04$ a	$11.32 ± 0.32 a$	$9.62 ± 0.75 b$	$0.29 ± 0.01 a$		$1.46 ± 0.03 a$	$1.38 ± 0.08 b$
间作加菌	Ⅰ	$6.27 ± 1.94 a$	$31.77 ± 1.70 a$	$43.47 ± 5.29 a$	$6.50 ± 2.16 a$	$12.60 ± 0.98 a$	$16.10 ± 2.01 a$	$0.08 ± 0.05 b$	$7.77 ± 0.46 b c$	$4.24 ± 0.55 c$	$0.16 ± 0.04 b$	$1.99 ± 0.13 d$	$1.65 ± 0.21 b$	$0.24 ± 0.09 b$	$9.76 ± 0.57 c$	$5.89 ± 0.76 b$	$0.11 ± 0.03 b$		$1.07 ± 0.01 c$	$0.79 ± 0.10 b$
	Ⅱ	$9.23 ± 2.04 a$	$28.57 ± 3.90 a$	$36.70 ± 3.60 a b$	$9.50 ± 2.73 a$	$11.48 ± 1.40 a b$	$12.13 ± 1.15 b$	$0.19 ± 0.08 b$	$6.60 ± 1.01 c$	$6.13 ± 0.59 b$	$0.35 ± 0.07 a$	$0.73 ± 0.09 e$	$3.83 ± 0.35 a$	$0.50 ± 0.15 a b$	$7.33 ± 1.08 d$	$9.96 ± 0.94 a$	$0.24 ± 0.05 a$		$0.93 ± 0.09 c$	$1.39 ± 0.13 a$
	Ⅲ	$7.90 ± 1.99 a$	$33.43 ± 0.84 a$	$32.73 ± 3.84 a b$	$8.57 ± 1.97 a$	$12.02 ± 0.65 a$	$11.80 ± 1.27 b$	$0.42 ± 0.25 a b$	$8.76 ± 0.19 b$	$4.35 ± 0.73 c$	$0.28 ± 0.05 a b$	$4.89 ± 0.54 a$	$2.02 ± 0.24 b$	$0.70 ± 0.29 a$	$13.65 ± 0.72 b$	$6.36 ± 0.97 b$	$0.18 ± 0.07 a b$		$1.61 ± 0.13 b$	$0.85 ± 0.17 b$
	Ⅳ	$6.10 ± 2.51 a$	$30.60 ± 0.45 a$	$40.10 ± 0.45 b$	$6.47 ± 2.04 a$	$11.75 ± 0.21 a b$	$16.07 ± 0.17 a$	$0.54 ± 0.15 a$	$6.47 ± 0.07 c$	$7.88 ± 0.03 a$	$0.25 ± 0.05 a b$	$2.89 ± 0.12 c$	$2.09 ± 0.05 b$	$0.79 ± 0.20 a$	$9.36 ± 0.14 c$	$9.97 ± 0.08 a$	$0.19 ± 0.05 a b$		$1.15 ± 0.03 c$	$1.42 ± 0.01 a$
	Ⅴ	$5.17 ± 0.46 a$	$28.33 ± 2.92 a$	$43.73 ± 2.46 a$	$6.17 ± 0.49 a$	$9.58 ± 0.97 b$	$18.23 ± 1.01 a$	$0.34 ± 0.03 a b$	$11.87 ± 0.50 a$	$5.12 ± 0.32 b c$	$0.24 ± 0.01 a b$	$3.83 ± 0.31 b$	$3.44 ± 0.18 a$	$0.5 ± 0.04 a b$	$15.70 ± 0.80 a$	$8.56 ± 0.50 a$	$0.17 ± 0.01 a b$		$1.98 ± 0.12 a$	$1.24 ± 0.07 a$

图1 不同种植模式下土壤pH变化注：同一种植模式下不同浓度梯度间的不同小写字母表示存在统计学差异（P<0.05）。

Fig. 1 Chang of soil pH under different planting patterns

图2 苜蓿在不同种植模式下不同组织重金属分布情况注：同一图内相同种植模式下不同浓度梯度的字母不同表示差异有统计学意义（P<0.05）。

Fig. 2 Distribution of heavy metals in different tissues under different planting patterns of alfalfa

图3 黑麦草在不同种植模式下不同组织重金属分布情况注：相同组织相同种植模式下不同浓度梯度的字母不同表示差异有统计学意义（P<0.05）。

Fig. 3 Distribution of heavy metals in different tissues under different planting patterns of ryegrass

图4 玉米在不同种植模式下不同组织重金属分布情况注：相同组织相同种植模式下不同浓度梯度的字母不同表示差异有统计学意义（P<0.05）。

Fig. 4 Distribution of heavy metals in different tissues under different planting patterns of maize

表4 3种植物在不同种植模式下的转运系数

Table 4 Transport coefficients of three plants under different planting patterns

重金属	浓度梯度	单作			间作			间作加菌
重金属	浓度梯度	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米
Zn	Ⅰ	$1.25 ± 0.17 a$	$0.59 ± 0.04 b$	$0.73 ± 0.04 b$	$1.94 ± 0.03 a$	$0.70 ± 0.02 a$	$0.87 ± 0.02 a$	$2.37 ± 0.28 a$	$0.59 ± 0.01 a$	$0.85 ± 0.01 a$
	Ⅱ	$0.45 ± 0.02 b$	$0.55 ± 0.02 b$	$1.19 ± 0.02 a$	$1.23 ± 0.02 b$	$0.45 ± 0.01 c$	$0.45 ± 0.01 c$	$0.83 ± 0.01 b$	$0.43 ± 0.02 d$	$0.30 ± 0.02 d$
	Ⅲ	$0.40 ± 0.03 b$	$0.48 ± 0.01 c$	$0.38 ± 0.01 d$	$0.42 ± 0.03 c$	$0.42 ± 0.01 c$	$0.43 ± 0.01 c$	$0.66 ± 0.05 b$	$0.50 ± 0.01 c$	$0.42 ± 0.01 c$
	Ⅳ	$0.33 ± 0.07 b$	$0.56 ± 0.02 b$	$0.66 ± 0.02 b c$	$0.32 ± 0.05 d$	$0.50 ± 0.01 b$	$0.53 ± 0.01 b$	$0.62 ± 0.03 b$	$0.53 ± 0.02 b c$	$0.51 ± 0.02 b$
	Ⅴ	$0.40 ± 0.02 b$	$0.77 ± 0.01 a$	$0.58 ± 0.01 c$	$0.47 ± 0.02 c$	$0.50 ± 0.01 b$	$0.36 ± 0.01 d$	$0.87 ± 0.04 b$	$0.55 ± 0.01 b$	$0.40 ± 0.01 c$
Cr	Ⅰ	$0.26 ± 0.01 a$	$N$	$N$	$0.21 ± 0.07 b$	$1.32 ± 0.11 a$	$N$	$0.81 ± 0.01 a$	$N$	$0.15 ± 0.03 b$
	Ⅱ	$0.33 ± 0.05 a$	$N$	$N$	$0.58 ± 0.04 a$	$0.18 ± 0.02 b$	$1.32 ± 0.02 a$	$0.71 ± 0.02 b$	$N$	$0.48 ± 0.03 a$
	Ⅲ	$0.31 ± 0.02 a$	$N$	$N$	$0.50 ± 0.02 a$	$0.15 ± 0.02 b$	$N$	$0.61 ± 0.02 c$	$N$	$0.001 ± 0.00 c$
	Ⅳ	$0.27 ± 0.07 a$	$N$	$N$	$0.53 ± 0.02 a$	$0.12 ± 0.02 b c$	$N$	$0.26 ± 0.02 d$	$0.02 ± 0.01 a$	$0.02 ± 0.01 c$
	Ⅴ	$0.11 ± 0.01 b$	$N$	$N$	$0.14 ± 0.02 b$	$N$	$N$	$0.13 ± 0.01 e$	$N$	$0.01 ± 0.00 c$
Cd	Ⅰ	$N$	$N$	$N$		$N$	$N$	N	$N$	$N$
	Ⅱ	$0.11 ± 0.02 b$	$3.14 ± 0.03 b$	$0.31 ± 0.00 c$	$0.16 ± 0.05 a$	$0.10 ± 0.03 c$	$0.14 ± 0.04 c$	$0.37 ± 0.01 a$	$0.12 ± 0.03 c$	$0.39 ± 0.13 b$
	Ⅲ	$0.09 ± 0.01 b$	$1.92 ± 0.19 c$	$0.60 ± 0.03 b c$	$0.09 ± 0.01 b$	$0.17 ± 0.02 b$	$0.24 ± 0.06 b$	$0.09 ± 0.02 b$	$0.18 ± 0.01 b$	$0.23 ± 0.03 c$
	Ⅳ	$0.16 ± 0.02 a$	$4.15 ± 0.09 a$	$0.99 ± 0.12 b$	$0.05 ± 0.01 b c$	$0.26 ± 0.02 a$	$0.48 ± 0.01 a$	$0.35 ± 0.02 a$	$0.22 ± 0.01 b$	$0.43 ± 0.04 b$
	Ⅴ	$0.19 ± 0.02 a$	$3.35 ± 0.09 b$	$2.07 ± 0.38 a$	$0.20 ± 0.03 a$	$0.24 ± 0.01 a$	$0.42 ± 0.04 a$	$0.29 ± 0.08 a$	$0.27 ± 0.01 a$	$0.67 ± 0.05 a$

表4 3种植物在不同种植模式下的转运系数

Table 4 Transport coefficients of three plants under different planting patterns

重金属	浓度梯度	单作			间作			间作加菌
重金属	浓度梯度	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米
Zn	Ⅰ	$1.25 ± 0.17 a$	$0.59 ± 0.04 b$	$0.73 ± 0.04 b$	$1.94 ± 0.03 a$	$0.70 ± 0.02 a$	$0.87 ± 0.02 a$	$2.37 ± 0.28 a$	$0.59 ± 0.01 a$	$0.85 ± 0.01 a$
	Ⅱ	$0.45 ± 0.02 b$	$0.55 ± 0.02 b$	$1.19 ± 0.02 a$	$1.23 ± 0.02 b$	$0.45 ± 0.01 c$	$0.45 ± 0.01 c$	$0.83 ± 0.01 b$	$0.43 ± 0.02 d$	$0.30 ± 0.02 d$
	Ⅲ	$0.40 ± 0.03 b$	$0.48 ± 0.01 c$	$0.38 ± 0.01 d$	$0.42 ± 0.03 c$	$0.42 ± 0.01 c$	$0.43 ± 0.01 c$	$0.66 ± 0.05 b$	$0.50 ± 0.01 c$	$0.42 ± 0.01 c$
	Ⅳ	$0.33 ± 0.07 b$	$0.56 ± 0.02 b$	$0.66 ± 0.02 b c$	$0.32 ± 0.05 d$	$0.50 ± 0.01 b$	$0.53 ± 0.01 b$	$0.62 ± 0.03 b$	$0.53 ± 0.02 b c$	$0.51 ± 0.02 b$
	Ⅴ	$0.40 ± 0.02 b$	$0.77 ± 0.01 a$	$0.58 ± 0.01 c$	$0.47 ± 0.02 c$	$0.50 ± 0.01 b$	$0.36 ± 0.01 d$	$0.87 ± 0.04 b$	$0.55 ± 0.01 b$	$0.40 ± 0.01 c$
Cr	Ⅰ	$0.26 ± 0.01 a$	$N$	$N$	$0.21 ± 0.07 b$	$1.32 ± 0.11 a$	$N$	$0.81 ± 0.01 a$	$N$	$0.15 ± 0.03 b$
	Ⅱ	$0.33 ± 0.05 a$	$N$	$N$	$0.58 ± 0.04 a$	$0.18 ± 0.02 b$	$1.32 ± 0.02 a$	$0.71 ± 0.02 b$	$N$	$0.48 ± 0.03 a$
	Ⅲ	$0.31 ± 0.02 a$	$N$	$N$	$0.50 ± 0.02 a$	$0.15 ± 0.02 b$	$N$	$0.61 ± 0.02 c$	$N$	$0.001 ± 0.00 c$
	Ⅳ	$0.27 ± 0.07 a$	$N$	$N$	$0.53 ± 0.02 a$	$0.12 ± 0.02 b c$	$N$	$0.26 ± 0.02 d$	$0.02 ± 0.01 a$	$0.02 ± 0.01 c$
	Ⅴ	$0.11 ± 0.01 b$	$N$	$N$	$0.14 ± 0.02 b$	$N$	$N$	$0.13 ± 0.01 e$	$N$	$0.01 ± 0.00 c$
Cd	Ⅰ	$N$	$N$	$N$		$N$	$N$	N	$N$	$N$
	Ⅱ	$0.11 ± 0.02 b$	$3.14 ± 0.03 b$	$0.31 ± 0.00 c$	$0.16 ± 0.05 a$	$0.10 ± 0.03 c$	$0.14 ± 0.04 c$	$0.37 ± 0.01 a$	$0.12 ± 0.03 c$	$0.39 ± 0.13 b$
	Ⅲ	$0.09 ± 0.01 b$	$1.92 ± 0.19 c$	$0.60 ± 0.03 b c$	$0.09 ± 0.01 b$	$0.17 ± 0.02 b$	$0.24 ± 0.06 b$	$0.09 ± 0.02 b$	$0.18 ± 0.01 b$	$0.23 ± 0.03 c$
	Ⅳ	$0.16 ± 0.02 a$	$4.15 ± 0.09 a$	$0.99 ± 0.12 b$	$0.05 ± 0.01 b c$	$0.26 ± 0.02 a$	$0.48 ± 0.01 a$	$0.35 ± 0.02 a$	$0.22 ± 0.01 b$	$0.43 ± 0.04 b$
	Ⅴ	$0.19 ± 0.02 a$	$3.35 ± 0.09 b$	$2.07 ± 0.38 a$	$0.20 ± 0.03 a$	$0.24 ± 0.01 a$	$0.42 ± 0.04 a$	$0.29 ± 0.08 a$	$0.27 ± 0.01 a$	$0.67 ± 0.05 a$

表5 三种植物在不同种植模式下的富集系数

Table 5 Enrichment coefficients of three plants under different planting patterns

重金属	浓度梯度	单作			间作			间作加菌
重金属	浓度梯度	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米
Zn	Ⅰ	$0.35 ± 0.05 a$	$0.35 ± 0.02 d$	$0.40 ± 0.03 b$	$0.53 ± 0.01 a$	$0.75 ± 0.02 a$	$0.71 ± 0.04 a$	$0.65 ± 0.07 b$	$1.01 ± 0.02 a$	$0.72 ± 0.01 a$
	Ⅱ	$0.32 ± 0.01 a$	$0.63 ± 0.02 a$	$0.46 ± 0.04 a$	$0.46 ± 0.01 b$	$0.66 ± 0.02 b$	$0.51 ± 0.02 b$	$0.48 ± 0.01 c$	$0.67 ± 0.04 b$	$0.62 ± 0.01 b$
	Ⅲ	$0.28 ± 0.02 a b$	$0.53 ± 0.01 b$	$0.22 ± 0.01 c$	$0.38 ± 0.03 c$	$0.41 ± 0.01 c$	$0.35 ± 0.01 c$	$0.65 ± 0.05 b$	$0.56 ± 0.01 c$	$0.36 ± 0.01 d$
	Ⅳ	$0.26 ± 0.05 a b$	$0.51 ± 0.02 b$	$0.35 ± 0.01 b$	$0.25 ± 0.04 e$	$0.41 ± 0.01 c$	$0.37 ± 0.01 c$	$0.51 ± 0.03 c$	$0.52 ± 0.02 c$	$0.40 ± 0.02 c$
	Ⅴ	$0.22 ± 0.01 b$	$0.50 ± 0.01 b$	$0.28 ± 0.03 c$	$0.32 ± 0.02 d$	$0.40 ± 0.01 c$	$0.26 ± 0.01 d$	$0.79 ± 0.04 a$	$0.51 ± 0.01 c$	$0.35 ± 0.02 d$
Cr	Ⅰ	$0.38 ± 0.02 a$	$N$	$N$	$0.78 ± 0.28 a$	$5.66 ± 0.46 a$	$N$	$2.29 ± 0.02 a$	$N$	$0.41 ± 0.08 b$
	Ⅱ	$0.10 ± 0.01 c$	$N$	$N$	$0.19 ± 0.01 b$	$0.14 ± 0.02 b$	$0.07 ± 0.01 a$	$0.59 ± 0.02 b$	$N$	$1.31 ± 0.08 a$
	Ⅲ	$0.19 ± 0.01 b$	$N$	$N$	$0.32 ± 0.02 b$	$0.06 ± 0.01 b$	$N$	$0.48 ± 0.02 c$	$N$	$0.001 ± 0.00 c$
	Ⅳ	$0.03 ± 0.01 d$	$N$	$N$	$0.27 ± 0.01 b$	$0.06 ± 0.01 b$	$N$	$0.09 ± 0.01 d$	$0.02 ± 0.01 a$	$0.01 ± 0.00 c$
	Ⅴ	$0.08 ± 0.01 c$	$N$	$N$	$0.08 ± 0.01 b$	$N$	$N$	$0.09 ± 0.01 d$	$N$	$0.01 ± 0.00 c$
Cd	Ⅰ	$N$	$N$	$N$	$N$	$N$	$N$	$N$	$N$	$N$
	Ⅱ	$0.37 ± 0.02 b$	$3.14 ± 0.03 b$	$1.93 ± 0.02 b c$	$0.22 ± 0.07 b$	$0.43 ± 0.14 b$	$0.83 ± 0.22 b$	$0.64 ± 0.03 c$	$0.96 ± 0.26 b$	$1.85 ± 0.62 b c$
	Ⅲ	$0.42 ± 0.04 b$	$1.92 ± 0.19 c$	$1.20 ± 0.05 c$	$0.22 ± 0.02 b$	$0.44 ± 0.26 b$	$0.49 ± 0.12 b$	$0.23 ± 0.06 d$	$1.55 ± 0.05 a$	$1.10 ± 0.15 c$
	Ⅳ	$0.82 ± 0.13 a$	$4.15 ± 0.09 a$	$2.72 ± 0.33 b$	$0.17 ± 0.03 b c$	$1.36 ± 0.12 a$	$2.87 ± 0.06 a$	$1.41 ± 0.09 a$	$1.83 ± 0.11 a$	$2.88 ± 0.28 a$
	Ⅴ	$0.96 ± 0.13 a$	$3.35 ± 0.09 b$	$4.02 ± 0.73 a$	$1.08 ± 0.18 a$	$1.32 ± 0.06 a$	$2.68 ± 0.27 a$	$0.96 ± 0.25 b$	$1.16 ± 0.00 b$	$2.15 ± 0.17 a b$

表5 三种植物在不同种植模式下的富集系数

Table 5 Enrichment coefficients of three plants under different planting patterns

重金属	浓度梯度	单作			间作			间作加菌
重金属	浓度梯度	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米	苜蓿	黑麦草	玉米
Zn	Ⅰ	$0.35 ± 0.05 a$	$0.35 ± 0.02 d$	$0.40 ± 0.03 b$	$0.53 ± 0.01 a$	$0.75 ± 0.02 a$	$0.71 ± 0.04 a$	$0.65 ± 0.07 b$	$1.01 ± 0.02 a$	$0.72 ± 0.01 a$
	Ⅱ	$0.32 ± 0.01 a$	$0.63 ± 0.02 a$	$0.46 ± 0.04 a$	$0.46 ± 0.01 b$	$0.66 ± 0.02 b$	$0.51 ± 0.02 b$	$0.48 ± 0.01 c$	$0.67 ± 0.04 b$	$0.62 ± 0.01 b$
	Ⅲ	$0.28 ± 0.02 a b$	$0.53 ± 0.01 b$	$0.22 ± 0.01 c$	$0.38 ± 0.03 c$	$0.41 ± 0.01 c$	$0.35 ± 0.01 c$	$0.65 ± 0.05 b$	$0.56 ± 0.01 c$	$0.36 ± 0.01 d$
	Ⅳ	$0.26 ± 0.05 a b$	$0.51 ± 0.02 b$	$0.35 ± 0.01 b$	$0.25 ± 0.04 e$	$0.41 ± 0.01 c$	$0.37 ± 0.01 c$	$0.51 ± 0.03 c$	$0.52 ± 0.02 c$	$0.40 ± 0.02 c$
	Ⅴ	$0.22 ± 0.01 b$	$0.50 ± 0.01 b$	$0.28 ± 0.03 c$	$0.32 ± 0.02 d$	$0.40 ± 0.01 c$	$0.26 ± 0.01 d$	$0.79 ± 0.04 a$	$0.51 ± 0.01 c$	$0.35 ± 0.02 d$
Cr	Ⅰ	$0.38 ± 0.02 a$	$N$	$N$	$0.78 ± 0.28 a$	$5.66 ± 0.46 a$	$N$	$2.29 ± 0.02 a$	$N$	$0.41 ± 0.08 b$
	Ⅱ	$0.10 ± 0.01 c$	$N$	$N$	$0.19 ± 0.01 b$	$0.14 ± 0.02 b$	$0.07 ± 0.01 a$	$0.59 ± 0.02 b$	$N$	$1.31 ± 0.08 a$
	Ⅲ	$0.19 ± 0.01 b$	$N$	$N$	$0.32 ± 0.02 b$	$0.06 ± 0.01 b$	$N$	$0.48 ± 0.02 c$	$N$	$0.001 ± 0.00 c$
	Ⅳ	$0.03 ± 0.01 d$	$N$	$N$	$0.27 ± 0.01 b$	$0.06 ± 0.01 b$	$N$	$0.09 ± 0.01 d$	$0.02 ± 0.01 a$	$0.01 ± 0.00 c$
	Ⅴ	$0.08 ± 0.01 c$	$N$	$N$	$0.08 ± 0.01 b$	$N$	$N$	$0.09 ± 0.01 d$	$N$	$0.01 ± 0.00 c$
Cd	Ⅰ	$N$	$N$	$N$	$N$	$N$	$N$	$N$	$N$	$N$
	Ⅱ	$0.37 ± 0.02 b$	$3.14 ± 0.03 b$	$1.93 ± 0.02 b c$	$0.22 ± 0.07 b$	$0.43 ± 0.14 b$	$0.83 ± 0.22 b$	$0.64 ± 0.03 c$	$0.96 ± 0.26 b$	$1.85 ± 0.62 b c$
	Ⅲ	$0.42 ± 0.04 b$	$1.92 ± 0.19 c$	$1.20 ± 0.05 c$	$0.22 ± 0.02 b$	$0.44 ± 0.26 b$	$0.49 ± 0.12 b$	$0.23 ± 0.06 d$	$1.55 ± 0.05 a$	$1.10 ± 0.15 c$
	Ⅳ	$0.82 ± 0.13 a$	$4.15 ± 0.09 a$	$2.72 ± 0.33 b$	$0.17 ± 0.03 b c$	$1.36 ± 0.12 a$	$2.87 ± 0.06 a$	$1.41 ± 0.09 a$	$1.83 ± 0.11 a$	$2.88 ± 0.28 a$
	Ⅴ	$0.96 ± 0.13 a$	$3.35 ± 0.09 b$	$4.02 ± 0.73 a$	$1.08 ± 0.18 a$	$1.32 ± 0.06 a$	$2.68 ± 0.27 a$	$0.96 ± 0.25 b$	$1.16 ± 0.00 b$	$2.15 ± 0.17 a b$

图5 3种植物不同种植模式下的叶绿素含量

Fig. 5 Chlorophyll content of the three plants under different planting modes

参考文献 23

1	曹金亮,王润福,张建萍,等.山西省矿山环境地质问题及其研究现状[J].地质通报,2004,23(11):1119-1126.
2	林嘉意,谢炬,朱越平,等.代谢组学及其在环境毒理学中的应用[J].生物技术进展,2022,12(5):683-689.
3	赵红芳,黄宝康.重金属胁迫下药用植物耐受及超富集的机制[J].药学实践杂志,2010,28(6):406-409.
4	董馨岚.高羊茅、黑麦草对镉、锌复合污染土壤的修复潜力研究[D].金华:浙江师范大学,2020.
5	王兴利,王晨野,吴晓晨,等.重金属污染土壤修复技术研究进展[J].化学与生物工程,2019,36(2): 1-7+11.
6	杨卓,王占利,李博文,等.微生物对植物修复重金属污染土壤的促进效果[J].应用生态学报,2009,20(8):2025-2031.
7	游梦,邹茸,王丽,等.不同富集植物与小麦间作对镉吸收转运的影响[J].中国土壤与肥料,2022(4):201-208.
8	肖宇,刘青松,徐玉鹏,等.苜蓿-玉米-小黑麦"草粮兼顾"型种植模式产量及经济效益分析[J].中国农学通报,2018,34(33):17-20.
9	张洁,张崇玉,张桂国.苜蓿/玉米-黑麦草连茬间作栽培提高牧草的产量、营养品质和降解性[C]//第七届(2017)中国苜蓿发展大会论文集,2017:319-328.
10	张晓宇.镉、镍单一/复合胁迫对紫花苜蓿种子及幼苗的影响研究[D].沈阳:沈阳化工大学,2021.
11	徐卫红,王宏信,王正银,等.重金属富集植物黑麦草对锌、镉复合污染的响应[J].中国农学通报,2006,22(6):365-368.
12	徐彦红.三种草本植物对铅-锌-镉污染的修复效果比较研究[D].贵州:贵州大学,2019.
13	房增国,赵秀芬,孙建好,等.接种根瘤菌对蚕豆/玉米间作系统产量及结瘤作用的影响[J].土壤学报,2009,46(5):887-893.
14	何国兴,宋建超,温雅洁,等.不同根瘤菌肥对紫花苜蓿生产力及土壤肥力的综合影响[J].草业学报,2020,29(5):109-120.
15	闫志强,陈银萍,蘧苗苗,等.镉胁迫对紫花苜蓿幼苗生理特性和镉富集的影响[J].广西植物,2019,39(2):218-227.
16	邹文桐,王艳君,曹智,等.不同种植模式下重金属污染对牧草叶片光合特性和重金属含量的影响[J].热带亚热带植物学报,2021,29(1):31-40.
17	CUI T T, FANG L C, WANG M K, et al.. Intercropping of Gramineous Pasture Ryegrass (Lolium perenne L.) and Leguminous Forage Alfalfa (Medicago sativa L.) increases the resistance of plants to heavy metals[J]. J. Chem., 2018(2018): 1-11.
18	张建华,马义勇,王振南,等.间作系统中玉米光合作用指标改善的研究[J].玉米科学,2006,14(4):104-106.
19	荣良燕,姚拓,刘青海,等.复合菌肥代替部分化肥对玉米生长的影响[J].草原与草坪, 2012,32(3):65-69.
20	ZHANG C l, CHEN Y Q, XU W H, et al.. Resistance of alfalfa and Indian mustard to Cd and the correlation of plant Cd uptake and soil Cd form[J]. Environ. Sci. Pollu. Res. Int, 2018, 26(14): 13804-13811.
21	沈佳怡,高铭晶,刘钰,等.典型草本植物对Cd-Zn-Cu污染河道底泥的修复[J].上海大学学报(自然科学版),2022,28(1):40-48.
22	杜瑞英,文典,赵迪,等.Cd、Pb和As在不同品种水稻籽粒中的富集特征研究[J].生物技术进展,2016,6(2):85-90.
23	李文学,陈同斌.超富集植物吸收富集重金属的生理和分子生物学机制[J].应用生态学报,2003,14(4):627-631.

[1]	王振, YAO Mawulikplimi Adzavon, 刘梓嘉, 刘梦昱, 谢飞, 马雪梅, 赵鹏翔. 胎儿早期小鼠无疤痕愈合的数据转录组学分析[J]. 生物技术进展, 2023, 13(1): 131-139.
[2]	曹丹, 马林龙, 刘艳丽, 王丽丽, 金孝芳. 植物营养元素胁迫相关microRNA研究进展[J]. 生物技术进展, 2022, 12(6): 801-805.
[3]	林嘉意, 谢炬, 朱越平, 谢文玉. 代谢组学及其在环境毒理学中的应用[J]. 生物技术进展, 2022, 12(5): 683-689.
[4]	刘一超, 陆超, 战嵛华, 柯秀彬, 陆伟, 燕永亮. 施氏假单胞菌A1501铁吸收调节蛋白Fur的表达特性与功能鉴定[J]. 生物技术进展, 2022, 12(3): 387-395.
[5]	都润, 张思琦, 张海文, 陈涛. 逆境胁迫下向日葵的耐受机制[J]. 生物技术进展, 2022, 12(2): 205-212.
[6]	张融雪,孙玥,苏京平,王胜军,刘燕清,佟卉,孙林静. 植物内质网胁迫研究进展[J]. 生物技术进展, 2021, 11(3): 289-297.
[7]	张融雪,孙玥,苏京平,王胜军,佟卉,刘燕清,孙林静. 植物褪黑素研究进展[J]. 生物技术进展, 2021, 11(3): 297-303.
[8]	黄诗颖,谭景艾,王鹏,蒋宁飞,贺浩华,,边建民,. 以回交重组自交系定位水稻萌芽期耐镉胁迫相关QTLs[J]. 生物技术进展, 2021, 11(2): 176-181.
[9]	张香香,滕炎桐,陈涛. AtEXD参与植物盐胁迫的作用研究[J]. 生物技术进展, 2021, 11(1): 61-68.
[10]	杨文平,,吴远根. 基于Fe₃O₄过氧化物酶活性的Cd²⁺和Pb²⁺比色传感器[J]. 生物技术进展, 2019, 9(6): 611-619.
[11]	张在宝,,赵海,胡梦辉,邓丽君,王琦,李九丽,袁红雨,. 组学在花药发育研究中的应用进展Ⅰ:转录组学[J]. 生物技术进展, 2019, 9(5): 433-439.
[12]	李丽,刘双清,杨远航,戴良英,李魏. 热激转录因子在植物抗非生物胁迫中的功能研究进展[J]. 生物技术进展, 2018, 8(3): 214-220.
[13]	陈新兵,黄荣峰,王娟. 水稻矮杆突变体D814基因图位克隆与功能分析[J]. 生物技术进展, 2017, 7(6): 608-617.
[14]	沈少炎,吴玉香,郑郁善,. 植物干旱胁迫响应机制研究进展——从表型到分子[J]. 生物技术进展, 2017, 7(3): 169-176.
[15]	许博思,孟红岩,张春义,姜凌. 拟南芥质体定位的叶酰谷氨酸合成酶基因在低氮条件下的功能分析[J]. 生物技术进展, 2017, 7(1): 30-37.

3种植物对复合重金属的胁迫效应及富集转运特征

Stress Effects， Enrichment and Transport Characteristics of Three Plants on Complex Heavy Metals

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 23

相关文章 15

编辑推荐

Metrics

本文评价