苯二酚内酯及其衍生物杀线虫活性研究

doi:10.19586/j.2095-2341.2017.0026

生物技术进展 ›› 2017, Vol. 7 ›› Issue (6): 632-638.DOI: 10.19586/j.2095-2341.2017.0026

苯二酚内酯及其衍生物杀线虫活性研究

张荣儒1，2，3,张礼文1,张鹏1,章跃陵2,刘柱3*,徐玉泉1*

1.中国农业科学院生物技术研究所, 北京 100081; 2.汕头大学理学院, 广东汕头 515063； 3.海南大学热带农林学院, 海口 570228

收稿日期:2017-04-13 出版日期:2017-11-25 发布日期:2017-06-14
通讯作者: 刘柱，教授，主要从事微生物农药研究。E-mail:zhuliu@hainu.edu.cn;徐玉泉，研究员，主要从事微生物次生代谢产物的研究。E-mail:xuyuquan@caas.cn
作者简介:张荣儒，硕士研究生，主要从事微生物防治植物病害研究。E-mail:zzru2010@126.com。
基金资助:
国家自然科学基金项目（31560021）；农业部948计划项目（2016-X43）；国家国际科技合作专项（2015DFR31060）；海南省重点研发计划（ZDYF2017020）资助。

Studies on the Nematocidal Activities of Benzenediol Lactones and Their Derivatives

ZHANG Rongru,, ZHANG Liwen, ZHANG Peng, ZHANG Yueling, LIU Zhu, XU Yuquan

1.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China2.College of Science, Shantou University, Guangdong Shantou 515063, China; 3.Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China

Received:2017-04-13 Online:2017-11-25 Published:2017-06-14

摘要/Abstract

摘要： 采用浸渍法研究了酿酒酵母异源表达的苯二酚内酯monocillin II、10,11-dehydrocurvularin、pre-asperfuranone、trans-resorcylide、desmethyl-lasiodiplodin和lasiodiplodin的杀秀丽线虫活性。首次发现苯二酚内酯10,11-dehydrocurvularin和desmethyl-lasiodiplodin具有杀线虫活性。分别以0.1 mg/mL、0.2 mg/mL 和0.5 mg/mL的10,11-dehydrocurvularin和desmethyl-lasiodiplodin处理秀丽线虫的二龄幼虫（L2）24 h后，秀丽线虫的校正死亡率分别为7.95%、15.30%、46.41%和3.41%、8.90%、27.47%。Desmethyl-lasiodiplodin的甲基化产物lasiodiplodin的杀线虫活性降低，浓度为0.5 mg/mL时，线虫的校正死亡率为16.12%。研究结果为植物线虫病害的防治提供了新的思路。

关键词: 苯二酚内酯, 秀丽线虫, 10,11-dehydrocurvularin, desmethyl-lasiodiplodin, 杀线虫活性

Abstract: Benzenediol lactones(BDLs) and their derivatives, including monocillin II, 10,11-dehydrocurvularin, pre-asperfuranone, trans-resorcylide, desmethyl-lasiodiplodin and lasiodiplodin， were obtained by heterologous biosynthesis in yeast. Their nematocidal activities were researched in this study. For the first time, 10,11-dehydrocurvularin and desmethyl-lasiodiplodin were found with nematocidal activity. After treating Caenorhabditis elegans with 0.1 mg/mL, 0.2 mg/mL and 0.5 mg/mL of 10,11-dehydrocurvularin and desmethyl-lasiodiplodin for 24 h, respectively, the corrected mortality rate of C. elegans was 7.95%, 15.30%, 46.41% and 3.41%, 8.90%, 27.47%. Nematocidal activity of lasiodiplodin was decreased, and the corrected mortality rate with 0.5 mg/mL was 16.12%. This study provided a new idea for the prevention and cure of plant nematode disease.

Key words: benzenediol lactones, Caenorhabditis elegans, 10,11-dehydrocurvularin, desmethyl-lasiodiplodin, nematicidal activity

张荣儒,,张礼文,张鹏,章跃陵,刘柱,徐玉泉. 苯二酚内酯及其衍生物杀线虫活性研究[J]. 生物技术进展, 2017, 7(6): 632-638.

ZHANG Rongru1,2,3, ZHANG Liwen1, ZHANG Peng1, ZHANG Yueling2, LIU Zhu3*, XU Yuquan1*. Studies on the Nematocidal Activities of Benzenediol Lactones and Their Derivatives[J]. Curr. Biotech., 2017, 7(6): 632-638.

[1]	王晓婧,陆伟,徐玉泉,梁晓东. 真菌苯二酚内酯聚酮类化合物生物合成研究进展 [J]. 生物技术进展, 2015, 5(2): 89-94.
[2]	白净,陆伟,徐玉泉,陈明. 苯二酚内酯合成途径中的聚酮合酶组合表达研究[J]. 生物技术进展, 2015, 5(1): 54-59.

苯二酚内酯及其衍生物杀线虫活性研究

Studies on the Nematocidal Activities of Benzenediol Lactones and Their Derivatives

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