利用宏基因组法筛选新几丁质酶基因

doi:10.3969/j.issn.2095-2341.2016.02.07

生物技术进展 ›› 2016, Vol. 6 ›› Issue (2): 119-124.DOI: 10.3969/j.issn.2095-2341.2016.02.07

利用宏基因组法筛选新几丁质酶基因

王海东1,胡忠2*

1.徐州生物工程职业技术学院, 江苏徐州 221006;

2.汕头大学生物系, 广东汕头 515000

收稿日期:2015-08-14 出版日期:2016-03-25 发布日期:2015-09-15
通讯作者: 胡忠，教授，博士生导师，研究方向为生物技术。 E-mail:hzh@stu.edu.cn
作者简介:王海东，硕士研究生，研究方向为生物技术。E-mial: wanghaidong6@163.com。

Screening of New Chitinase Genes with Metagenomics

WANG Hai-dong, HU Zhong

1.Xuzhou Vocational College of Bioengineering,

Jiangsu Xuzhou 221006, China;

2.Department of Biology, Shantou University,

Guangdong Shantou 515000, China

Received:2015-08-14 Online:2016-03-25 Published:2015-09-15

摘要/Abstract

摘要： 为了寻找新型几丁质酶编码基因，提取了汕头湾海域海底表层沉积物中微生物的宏基因组，采用PCR-DGGE技术扩增和分离获得了新型几丁质酶基因编码信息。实验共得到63条几丁质酶基因片段，其编码的蛋白序列与NCBI 数据库收录的序列相似率在41%～97%之间，且大多数在 70%以下，只有8条相似率在70%以上，NCBI数据库中与其相似性最高的几丁质酶蛋白序列有18个种属，其中与橙色滑柱菌株 (Herpetosiphon aurantiacus) ATCC 23779 的几丁质酶存在着最高相似性的序列有27条，另外，与未可培养细菌几丁质酶基因片段相似的有22条，占34.9% ，分属9个种属。说明汕头湾表层沉积物中存在多种几丁质酶编码基因，其中有很多是尚未研究的，为寻找新型的几丁质酶编码基因提供了有力的生物资源。

关键词: 汕头湾, 几丁质酶, DGGE, 宏基因组, 表层沉积物

Abstract: In order to find new chitinease-encoding genes, we extracted metagenome from surface sediments of the Shantou Bay, and amplified 63 chitinase coding gene fragments using PCR-DGGE. Compared these fragments to NCBI records, the similarity ratios were from 41% to 97%. The most similarity ratios were below 70%, only 8 higher than 70%, and there were 18 species in NCBI chitinase records had the highest similarity ratios. 27 fragments had the highest similarity ratios to the chitinase sequence of Herpetosiphon aurantiacus ATCC 23779. 22 fragments had the highest similarity ratios to uncultured bacteria chitinase sequences which belonged to 9 different species, accounted for 34.9%. The results suggested that there were many different chitinase-coding genes in Shantou Bay sediments, most of them hadn’t yet been studied from now. The paper provided a biological resources for finding new chitinease-encoding genes.

Key words:

Shantou Bay, chitinase, DGGE, metagenome, surface

sediment

王海东,胡忠. 利用宏基因组法筛选新几丁质酶基因[J]. 生物技术进展, 2016, 6(2): 119-124.

WANG Hai-dong1, HU Zhong2*. Screening of New Chitinase Genes with Metagenomics[J]. Curr. Biotech., 2016, 6(2): 119-124.

[1]	李伟, 王冲, 刘嗣嘉, 杨敏一, 张云平. 宏基因组学技术在痤疮研究中的应用进展[J]. 生物技术进展, 2021, 11(6): 694-699.
[2]	董俊帅,秦星,张志伟,张宇宏,张伟. 宏基因组来源果胶酸裂解酶在毕赤酵母中的表达及应用[J]. 生物技术进展, 2018, 8(3): 237-245.
[3]	吴森,张莺莺,昝林森, . 基于高通量测序的宏基因组学技术在动物胃肠道微生物方面的研究进展[J]. 生物技术进展, 2015, 5(2): 77-84.

利用宏基因组法筛选新几丁质酶基因

Screening of New Chitinase Genes with Metagenomics

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价