生物技术进展 ›› 2016, Vol. 6 ›› Issue (2): 125-129.DOI: 10.3969/j.issn.2095-2341.2016.02.08

• 研究论文 • 上一篇    下一篇

柚皮中黄酮类化合物提取工艺研究

李歌1,王珊珊1*,丁文珺1,牛佳1,姚倩祺2   

  1. 1.沈阳工学院生命工程学院, 辽宁 抚顺 113122;
    2.辽宁省抚顺市兽药饲料监察所, 辽宁 抚顺 113122
  • 收稿日期:2015-09-08 出版日期:2016-03-25 发布日期:2015-09-21
  • 通讯作者: 王珊珊,讲师,研究方向为生化与微生物发酵。E-mail:shuihugai@163.com
  • 作者简介:李歌,本科生,研究方向为天然活性成分的提取及应用。E-mail:1324265495@qq.com。
  • 基金资助:

    辽宁省教育规划课题(JG14DB310)资助。

Research on Extraction Process of Flavonoids From Pomelo Peel

LI Ge, WANG Shan-shan, DING Wen-jun, NIU Jia, YAO Qian-qi   

  1. 1.Life Engineering College of Shenyang Institute
    of Technology, Liaoning Fushun 113122, China;
    2.Fushun Institute of Veterinary Drug Control of
    Liaoning Province, Liaoning Fushun 113122, China
  • Received:2015-09-08 Online:2016-03-25 Published:2015-09-21

摘要: 柚子的果皮中含有果胶、膳食纤维、香精油、多糖、黄 酮类化合物等多种活性成分,具有很高的药用和食用价 值。通过对柚子皮资源进行合理的开发利用和深加工, 不仅能够减少环境污染,还能创造较好的经济效益和社 会效益。采用超声波法对柚皮中黄酮类物质进行提取, 研究了柚皮中黄酮提取过程中的影响因素,并通过单因 素试验和正交试验法以及验证试验对提取过程中的工艺 参数进行探究,最终确定最佳提取工艺为:乙醇体积分 数为80%,超声时间30 min,浸泡温度60℃,料液比 1∶25,在此条件下,柚皮中黄酮得率为4.63%。

关键词: 柚子皮, 黄酮类化合物, 超声波法

Abstract: Pomelo peel contains many kinds of active ingredients, such as pecitin, dietary fiber, essential oil, polysaccharide and flavonoids, which has high medicinal and edible value. Reasonable development, utilization and deep processing using pomelo peel as material, could not only reduce the environment pollution, but also create good economic and social benefits. This experiment extracted flavonoids from the pomelo peel using the ultrasonic method, and studied the influence factors in the process of pomelo peel flavonoids extraction. Through the single factor and orthogonal experiment method to study the optimal extraction technological parameter, results showed that the optimal parameters were as follows: ethanol concentration was 80%, ultrasonic treatment time was 30 min, soaking temperature was 60℃, solid to liquid ratio was 1:25, and the yield of flavonoid was 4.63%.

Key words: pomelo peel, flavonoid, ultrasonic-assisted