肠道定植产KPC酶植生拉乌尔菌的耐药基因环境分析

doi:10.3969/j.issn.2095-2341.2013.0 .

生物技术进展 ›› 2013, Vol. 3 ›› Issue (5): 363-367.DOI: 10.3969/j.issn.2095-2341.2013.0 .

肠道定植产KPC酶植生拉乌尔菌的耐药基因环境分析

葛超荣

浙江大学医学院附属第一医院, 杭州 310003

收稿日期:2013-07-19 出版日期:2013-09-25 发布日期:2013-08-22
作者简介:葛超荣，副主任技师，主要从事医学微生物研究。E-mail：gechaorong@126.com
基金资助:
国家自然科学基金项目（81101283）资助。

Genetic Environment of bla_KPC-2 in the Intestinal Colonized Raoultella planticolas

GE Chao-rong

First Affiliated Hospital,College of Medicine, Zhejiang University, Hangzhou 310003, China

Received:2013-07-19 Online:2013-09-25 Published:2013-08-22

摘要/Abstract

摘要： 碳青霉烯酶编码基因的传播是临床抗菌治疗面临的严重威胁之一。采用PCR、Southern杂交、测序比对等方法筛查和研究了碳青霉烯类抗菌药物的相关耐药基因。首次在国内发现了产KPC酸的植生拉乌尔菌两株。分离到的植生拉乌尔菌对被测试的19种常用抗菌药物中的16种耐药，只对多粘菌素B、粘菌素和米诺环素敏感。它们均产KPC-2型碳青霉烯酶，其编码基因bla_KPC-2位于质粒上，两侧(包括bla_KPC-2)长度为7 498 bp的DNA序列所包含的结构从上游至下游分别为Tn3-transposase, Tn3-resolvase, ISKpn8, bla_KPC-2和ISKpn6-like元件。

关键词: 植生拉乌尔菌, 碳青霉烯酶, 质粒

Abstract: The increasing emergence and spread of Klebsiella pneumoniae carbapenemases (KPCs) leaves fewer available therapeutic options due to their broad-spectrum hydrolytic activity. Two KPC-2-producing Raoultella planticola isolates were first identified and analyzed using PCR, Southern hybridization and sequencing in China. Two isolates had an identical antimicrobial susceptibility profile, bla_KPC-2 gene as well as its flanking sequences. They were resistant to 16 antimicrobial agents while sensitive to polymyxin B, colistin and minocycline. The results demonstrated that the bla_KPC-2 gene was located on a 7 498 bp plasmid segment that encoded five open reading frames with the order of Tn3-transposase, Tn3-resolvase, ISKpn8, bla_KPC-2 gene and ISKpn6-like.

Key words: Raoultella planticola, carbapenemase, plasmid

葛超荣. 肠道定植产KPC酶植生拉乌尔菌的耐药基因环境分析[J]. 生物技术进展, 2013, 3(5): 363-367.

GE Chao-rong. Genetic Environment of bla_KPC-2 in the Intestinal Colonized Raoultella planticolas[J]. Curr. Biotech., 2013, 3(5): 363-367.

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肠道定植产KPC酶植生拉乌尔菌的耐药基因环境分析

Genetic Environment of bla_KPC-2 in the Intestinal Colonized Raoultella planticolas

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肠道定植产KPC酶植生拉乌尔菌的耐药基因环境分析

Genetic Environment of blaKPC-2 in the Intestinal Colonized Raoultella planticolas

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Genetic Environment of bla_KPC-2 in the Intestinal Colonized Raoultella planticolas