Kurthia huakuii LAM0618T重组酚氧化酶LaclK对乙基紫脱色的研究

doi:10.3969/j.issn.2095-2341.2016.04.06

生物技术进展 ›› 2016, Vol. 6 ›› Issue (4): 255-260.DOI: 10.3969/j.issn.2095-2341.2016.04.06

Kurthia huakuii LAM0618T重组酚氧化酶LaclK对乙基紫脱色的研究

郭翔1,2,阮志勇2*,宋金龙3,周珊2,王彦伟1,何明雄1,刘小秧2,胡国全1*

1.农业部沼气科学研究所, 农业部农村可再生能源开发利用重点实验室, 成都 610041;

2.中国农业科学院农业资源与农业区划研究所, 农业部农业微生物资源收集与保藏重点实验室, 北京 100081;

3.中国水产科学研究院, 北京 100141

收稿日期:2016-03-19 出版日期:2016-07-25 发布日期:2016-04-11
通讯作者: 胡国全，研究员，主要从事环境微生物学研究。E-mail：huguoquan1@hotmail.com；阮志勇，副研究员,博士，主要从事农业微生物资源发掘与利用研究。E-mail：ruanzhiyong@caas.cn
作者简介:郭翔，硕士研究生，主要从事环境微生物学研究。E-mail：gx20093879@163.com。
基金资助:
中央级公益性科研院所基本科研业务费专项资金（IARRP2014-30）;公益性行业（农业）科研专项（201403019）资助。

Decolorization of Ethyl Violet by Recombinant Phenol Oxidase LaclK from Kurthia huakuii LAM0618T

GUO Xiang, RUAN Zhi-yong, SONG Jin-long, ZHOU Shan, WANG Yan-wei, HE Ming-xiong, LIU Xiao-yang, HU Guo-quan

1.Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Biogas

Institute of Ministry of Agriculture, Chengdu 610041, China;

2.Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of

Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China;

3.Chinese Academy of Fishery Sciences, Beijing 100141, China

Received:2016-03-19 Online:2016-07-25 Published:2016-04-11

摘要/Abstract

摘要： 以三苯甲烷类染料乙基紫为研究对象，研究了Kurthia huakuii LAM0618T重组酚氧化酶LaclK对乙基紫的脱色性能，探讨了给酶量、脱色时间、染料浓度、反应pH、卤离子、有机试剂以及NO-3、SO2-4和腐殖酸对脱色效果的影响。在pH 7.0，60℃条件下反应1 h，200 U/L的LaclK对10 μmol/L和20 μmol/L的乙基紫显示出较好的脱色效果，脱色率分别达到91%和84%。此外， LaclK能够耐受卤离子和有机试剂，具有一定的生物修复应用潜力。

关键词: 乙基紫, LaclK, Kurthia huakuii LAM0618T, 酚氧化酶, 脱色

Abstract: The dye decolorization ability of recombinant LaclK on ethyl violet was studied in this work. Effects of parameters, such as amount of LaclK, incubation time, ethyl violet concentration, pH, halide ions, organic solvents, NO-3，SO2-4and humic acid on the decolorization was investigated. After incubation at 60℃ and pH 7.0 for 1 h, 200 U/L of LaclK decolorized 91% of 10 μmol/L ethyl violet and 84% of 20 μmol/L ethyl violet, respectively. Moreover, LaclK exhibited a strong tolerance to halide ions and organic solvents, indicating the industrial application potential on bioremediation.

Key words: ethyl violet, LaclK, Kurthia huakuii LAM0618T, phenol oxidase, decolorization

郭翔,阮志勇,宋金龙,周珊,王彦伟,何明雄,刘小秧,胡国全. Kurthia huakuii LAM0618T重组酚氧化酶LaclK对乙基紫脱色的研究[J]. 生物技术进展, 2016, 6(4): 255-260.

GUO Xiang1,2, RUAN Zhi-yong2*, SONG Jin-long3, ZHOU Shan2, WANG Yan-wei1, HE Ming-xiong1, LIU Xiao-yang2, HU . Decolorization of Ethyl Violet by Recombinant Phenol Oxidase LaclK from Kurthia huakuii LAM0618T[J]. Curr. Biotech., 2016, 6(4): 255-260.

Kurthia huakuii LAM0618T重组酚氧化酶LaclK对乙基紫脱色的研究

Decolorization of Ethyl Violet by Recombinant Phenol Oxidase LaclK from Kurthia huakuii LAM0618T

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