关键词: 灰葡萄孢, 抗药性, 分子检测方法

Abstract: Grey mould is caused by Botrytis cinerea infection, which is usually controlled by chemical methods. Along with wide usage of fungicides, resistance to fungicides occured frequently among B. cinerea. This paper summarized mutation sites associated with fungicide resistance in B. cinerea, involving 6 kinds of fungicides and 5 genes. This paper also reviewed molecular methods to detect fungicide resistant sites, such as sequencing method，CAPS，ARMS，Tetra primer ARMS-PCR, AS real-time PCR, ASPPAA PCR and LightCycler technology, etc.. Through analyzing and comparing different kinds of methods，problems in using these detection techniques were pointed out and development of high throughtput detection method was prospected in the future.

Key words: Botrytis cinerea, fungicide resistance, molecular detection methods