关键词: 热稳定性, 理性设计, 解折叠自由能

Abstract: The studies on the rational design methods for improving the protein thermalstability were the hotspots in the field of computational biology and protein engineering. Compared to the traditional methods for enhancing the protein thermostability, rational design methods had many advantages, such as high efficiency and strong purposes. Moreover, the methods could not only expand the application scope of enzymes, but also help us to understand the relationship between protein structure and its function. In this paper, we introduced some factors that related to the protein thermostability and strategies usually used to improve the protein thermostability in detail. However, there were many factors that could affect the thermalstability of proteins, and there were many intricate links among these factors. So far, the researchers did not yet propose a rational design strategy that could adapt to all proteins. But it was an important direction that need the computing biologists and protein engineering scientists to study.

Key words: thermostability, rational design, free energy of unfolding