p,p'-DDT在中华绒螯蟹体内的富集动力学研究

doi:10.19586/j.2095-2341.2024.0128

生物技术进展 ›› 2024, Vol. 14 ›› Issue (5): 848-856.DOI: 10.19586/j.2095-2341.2024.0128

• 研究论文 • 上一篇

p,p'-DDT在中华绒螯蟹体内的富集动力学研究

钟金清¹^,²^,³(), 胡鲲¹^,²^,³, 高晓宁¹^,²^,³, 房迪¹^,²^,³, 苏泽辉¹^,²^,³, 张思雨¹^,²^,³, 黄呈炜⁴()

^1.上海海洋大学，国家水生动物病原库，上海 201306
^2.上海海洋大学，水产科学国家级实验教学示范中心，上海 201306
^3.农业农村部淡水水产种质资源重点实验室，上海 201306
^4.宁波市海洋与渔业研究院，浙江宁波 315103

收稿日期:2024-07-15 接受日期:2024-07-14 出版日期:2024-09-25 发布日期:2024-10-22
通讯作者: 黄呈炜
作者简介:钟金清E-mail:1023490683@qq.com；
基金资助:
农产品质量安全风险评估项目(GJFP20230402);国家淡水水产种质资源库建设项目(FGRC18537)

In vivo Study of p,p'-DDT Bioconcentration Kinetics in Chinese Mitten Crab Eriocheir sinensis

Jinqing ZHONG¹^,²^,³(), Kun HU¹^,²^,³, Xiaoning GAO¹^,²^,³, Di FANG¹^,²^,³, Zehui SU¹^,²^,³, Siyu ZHANG¹^,²^,³, Chengwei HUANG⁴()

^1.National Aquatic Animal Pathogen Database，Shanghai Ocean University，Shanghai 201306，China
^2.National Experimental Teaching Demonstration Center of Aquatic Science，Shanghai Ocean University，Shanghai 201306，China
^3.Key Laboratory of Freshwater Aquatic Germplasm Resources，Ministry of Agriculture and Rural Affiairs，Shanghai 201306，China
^4.Ningbo Academy of Oceanology and Fishery，Zhejiang Ningbo 315103，China

Received:2024-07-15 Accepted:2024-07-14 Online:2024-09-25 Published:2024-10-22
Contact: Chengwei HUANG

摘要/Abstract

摘要：

为探究p,p'-滴滴涕（p,p'-dichlorodiphenyltrichloroethane, p,p'-DDT）在中华绒螯蟹（Eriocheir sinensis）体内的分布特征和生物富集动力学特点，基于双箱动力学模型，采用半静态水质接触染毒方式，通过对富集、释放阶段中华绒螯蟹肌肉、性腺和肝胰腺中p,p'-DDT含量的动态监测结果进行非线性拟合，得到了各组织器官的一级吸收速率常数k₁、一级释放速率常数k₂、生物富集系数BCF（bioconcentration factor）、富集平衡浓度C_A-SS、生物半衰期t₅₀等富集动力学参数。结果显示：各组织中k₁为0.021~16.039，k₂为0.102~0.191，BCF为0.146~83.974，C_A-SS为37.50~19 423.50 μg·kg^-1，t₅₀为3.63~6.80 d。模型拟合优势度检验结果显示：中华绒螯蟹在25、500 μg·L^-1的p,p'-DDT暴露下，肌肉、性腺、肝胰腺对p,p'-DDT的富集和释放过程符合双箱动力学模型。结果表明中华绒螯蟹肌肉、性腺和肝胰腺对p,p'-DDT的k₁和BCF随暴露浓度的增大而减小，C_A-SS均随暴露浓度的增大而增大，k₂和t₅₀则与暴露浓度无明显关联，不同组织对p,p'-DDT的富集效应强弱表现为肝胰腺>性腺>肌肉。

关键词: p,p'-DDT, 中华绒螯蟹, 生物富集, 双箱动力学模型

Abstract:

To investigate the distribution characteristics and bioconcentration kinetics of p,p'-dichlorodiphenyltri-chloroethane（p,p'-DDT） in Chinese mitten crab Eriocheir sinensis， the two-compartment kinetic model was employed. Using the semi-static water exposure method， the study conducted dynamic monitoring of p,p'-DDT concentrations in the muscle， gonad， and hepatopancreas of E. sinensis during both bioconcentration and depuration phases. Non-linear fitting was applied to these monitoring results to obtain various bioconcentration kinetic parameters for each tissue and organ， including first order constant for uptake （k₁）， first order rate constant for depuration （k₂）， bioconcentration factor （BCF）， concentration in the crab at steady-state （C_A-SS） and biological half-life （t₅₀）. Results among various tissues， k₁ was 0.021~16.039， k₂ was 0.102~0.191， BCF was 0.146~83.974， C_A-SS was 37.50~19 423.50 μg·kg ^-¹， t₅₀ was 3.63~6.80 d. Goodness-of-fit tests indicated that the bioconcentration and depuration processes of p,p'-DDT in muscle， gonad and hepatopancreas conformed to the two-compartment kinetic model while E. sinensis exposed to both 25 and 500 μg·L ^-¹p,p'-DDT. In details， k₁ and BCF in muscle， gonad and hepatopancreas decreased along with the increase of p,p'-DDT concentration. In contrast， C_A-SS increased as the concentration raised. Moreover， k₂ and t₅₀ displayed no significant correlation with the exposure concentration variation. As for the difference among tissues， the bioconcentration effect of p,p'-DDT in different tissues ranked as follows： hepatopancreas>gonad>muscle.

Key words: p,p'-DDT, Eriocheir sinensis, bioconcentration, two-compartment kinetic model

中图分类号:

Q959.9

钟金清, 胡鲲, 高晓宁, 房迪, 苏泽辉, 张思雨, 黄呈炜. p,p'-DDT在中华绒螯蟹体内的富集动力学研究[J]. 生物技术进展, 2024, 14(5): 848-856.

Jinqing ZHONG, Kun HU, Xiaoning GAO, Di FANG, Zehui SU, Siyu ZHANG, Chengwei HUANG. In vivo Study of p,p'-DDT Bioconcentration Kinetics in Chinese Mitten Crab Eriocheir sinensis[J]. Current Biotechnology, 2024, 14(5): 848-856.

图/表 7

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富集时间/d	25 μg·L^-1处理组			500 μg·L^-1处理组
富集时间/d	肌肉	性腺	肝胰腺	肌肉	性腺	肝胰腺
3	11.00±0.91c	26.40±0.70b	802.89±23.00a	21.14±1.71c	340.66±14.51b	4 922.38±223.16a
6	17.33±1.64c	59.00±2.53b	1 343.49±29.72a	39.46±2.11c	538.68±16.33b	10 597.36±1 463.78a
9	25.11±0.84c	88.50±2.77b	1 672.27±24.88a	50.54±1.55c	811.54±23.61b	13 598.74±802.09a
12	29.24±0.81c	106.54±5.19b	1 934.67±19.03a	64.58±2.49c	1 073.09±52.54b	15 896.04±798.01a
15	33.94±1.24c	110.27±2.39b	2 085.89±39.35a	65.77±2.80c	1 463.65±91.61b	17 926.55±870.97a

富集时间/d	25 μg·L^-1处理组			500 μg·L^-1处理组
富集时间/d	肌肉	性腺	肝胰腺	肌肉	性腺	肝胰腺
3	11.00±0.91c	26.40±0.70b	802.89±23.00a	21.14±1.71c	340.66±14.51b	4 922.38±223.16a
6	17.33±1.64c	59.00±2.53b	1 343.49±29.72a	39.46±2.11c	538.68±16.33b	10 597.36±1 463.78a
9	25.11±0.84c	88.50±2.77b	1 672.27±24.88a	50.54±1.55c	811.54±23.61b	13 598.74±802.09a
12	29.24±0.81c	106.54±5.19b	1 934.67±19.03a	64.58±2.49c	1 073.09±52.54b	15 896.04±798.01a
15	33.94±1.24c	110.27±2.39b	2 085.89±39.35a	65.77±2.80c	1 463.65±91.61b	17 926.55±870.97a

净化时间/d	25 μg·L^-1处理组			500 μg·L^-1处理组
净化时间/d	肌肉	性腺	肝胰腺	肌肉	性腺	肝胰腺
18	25.11±1.16c	90.28±1.83b	1 809.48±17.62a	43.56±2.49c	1 278.14±41.90b	8 159.24±72.80a
21	13.71±0.43c	53.3±1.74b	1 003.13±47.86a	36.53±1.54c	758.55±50.18b	6 189.01±70.41a
24	9.86±0.73c	36.69±1.33b	401.09±14.57a	14.09±0.86c	594.06±53.94b	3 813.78±125.72a
27	7.05±0.18c	23.88±2.74b	249.64±12.94a	8.01±1.02c	405.77±20.96b	2 800.36±148.05a
30	5.33±0.32b	12.86±2.86b	212.07±13.68a	4.18±0.83c	310.84±24.54b	1 908.81±160.06a

净化时间/d	25 μg·L^-1处理组			500 μg·L^-1处理组
净化时间/d	肌肉	性腺	肝胰腺	肌肉	性腺	肝胰腺
18	25.11±1.16c	90.28±1.83b	1 809.48±17.62a	43.56±2.49c	1 278.14±41.90b	8 159.24±72.80a
21	13.71±0.43c	53.3±1.74b	1 003.13±47.86a	36.53±1.54c	758.55±50.18b	6 189.01±70.41a
24	9.86±0.73c	36.69±1.33b	401.09±14.57a	14.09±0.86c	594.06±53.94b	3 813.78±125.72a
27	7.05±0.18c	23.88±2.74b	249.64±12.94a	8.01±1.02c	405.77±20.96b	2 800.36±148.05a
30	5.33±0.32b	12.86±2.86b	212.07±13.68a	4.18±0.83c	310.84±24.54b	1 908.81±160.06a

组织类型	处理组/（μg·L^-1）	R²	P值
肌肉	25	0.969	0.495
肌肉	500	0.952	0.718
性腺	25	0.953	0.267
性腺	500	0.876	0.992
肝胰腺	25	0.940	0.087
肝胰腺	500	0.992	0.019

p,p'-DDT在中华绒螯蟹体内的富集动力学研究

In vivo Study of p,p'-DDT Bioconcentration Kinetics in Chinese Mitten Crab Eriocheir sinensis

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组织器官	处理组/（μg·L^-1）	k₁	k₂	BCF	C_A-SS/（μg·kg^-1）	t₅₀/d
肌肉	25	0.189	0.126	1.500	37.50	5.50
肌肉	500	0.021	0.144	0.146	73.00	4.81
性腺	25	0.611	0.114	5.360	134.00	6.08
性腺	500	0.318	0.102	3.118	1 559.00	6.80
肝胰腺	25	16.039	0.191	83.974	2 099.35	3.63
肝胰腺	500	6.604	0.170	38.847	19 423.50	4.08