关键词: RNAi转基因植物, 安全监管, 脱靶效应, 沉默效率, 跨界调控

Abstract: RNA interference (RNAi) plays a vital role in the growth, development, anti-virus and transposon inactivation of plant, animal and fungi. At present, the RNAi technology has been successfully used to cultivate biotechnological products with excellent characteristics such as disease and insect resistance and quality improvement, which has provided strong support for the green development of agriculture. However, the relevant mechanism of RNAi has not been fully clarified yet, which makes transgenic plants based on RNAi technology face some problems that need to be solved urgently. At the same time, the safe and efficient supervision of the application of RNAi-based transgenic plants also needs to be further improved. Based on this situation, the current regulatory situation of RNAi-based transgenic plants and their breeding challenges were reviewed, and some suggestions for solving the existing problems were put forward, in order to provide new ideas for further application of RNAi technology in agricultural plant improvement and breeding, and provide basis for their regulatory evaluation.

Key words: RNAi-based transgenic plants, safety regulatory, off-target effect, silence efficiency, cross-kingdom regulation