关键词: 菌群结构, 幽门螺杆菌感染, 十二指肠溃疡, 菌群多样性

Abstract: Through the research on the structure of gastric mucosa in patients with Helicobacter pylori (Hp) positive and negative duodenal ulcer, the effects of Hp infection and duodenal ulcer on the structure of duodenal mucosa were investigated. Five cases of duodenal mucosa in patients with Hp-positive and Hp-negative duodenal ulcer were selected. The 16S rRNA gene amplicon sequencing was used to detect the proportion of Hp colonization in duodenal mucosa. Structural analysis was performed. Results showed that, the number of species in the Hp-positive mucosa of patients with duodenal ulcer was significantly higher than that of Hp-negative mucosa (P<0.05), but the difference in microbial diversity was not statistically significant. Except Hp, Prevotella and Neisseria were also the dominatnt flora.  The content of Leptotrichia was significantly different between the two groups (P<0.05). Results indicated that, Hp does not become the only dominant bacteria after infection of duodenal mucosa, but increases the number of species in the duodenal mucosa. And it has little effect on the microbial diversity and has a certain influence on the flora structure. Leptotrichia may be involved in the development of ulcers.

Key words: flora structure, Helicobacter pylori infection, duodenal ulcer, flora diversity