关键词: 人乳寡糖, 预处理, 分离分析, 结构表征

Abstract: Carbohydrate is one of the most important active substances in our life. It is not only the energy source for cells, but also used as the signal molecule participating in various activities in cells. Human milk oligosaccharides (HMOs) are the third most component in human milk, its quality are lower than those of lactose and lipids, and higher than that of proteins. HMOs play significant roles in babys development and health. Efficient analytical methods are the necessary prerequisite to a better understanding of the relationship between the oligosaccharide structures and biofunctions. This review provided a biref introduction of HMOs and discussed recent advances in the pretreatment, separation and analysis, structural characterization of HMOs.

Key words: human milk oligosaccharides, pretreatment, separation and analysis, structual characterization