难培养微生物培养方法的研究进展

doi:10.3969/j.issn.2095-2341.2012.0 .

生物技术进展 ›› 2012, Vol. 2 ›› Issue (3): 165-170.DOI: 10.3969/j.issn.2095-2341.2012.0 .

难培养微生物培养方法的研究进展

曾建民,曾振顺,原红娟,谭志远*

华南农业大学农学院, 广州 510642

收稿日期:2012-03-22 出版日期:2012-05-25 发布日期:2012-04-14
通讯作者: 谭志远,教授，博士，主要从事微生物遗传学研究。E-mail:zytan@scau.edu.cn
作者简介:曾建民,硕士研究生,研究方向为固氮微生物的分离鉴定。
基金资助:
高校博士点专项基金（20094404110006）；国家973计划项目（2010CB126502）；广东省科技厅项目(2010B060200017，2010B090400450)资助。

Advances in Studies of Culture Methods of Oligotrophic Microbes

ZENG Jian-min, ZENG Zhen-shun, YUAN Hong-juan, TAN Zhi-yuan

College of Agriculture, South China Agricultural University, Guangzhou 510642, China

Received:2012-03-22 Online:2012-05-25 Published:2012-04-14

摘要/Abstract

摘要： 利用环境样品16S rRNA克隆测序等许多非培养(culture-independent)研究表明,自然界存在许多独特而难培养未培养的微生物,如何开发更多的微生物资源成为当前重要的课题,本文综述了影响分离得到难培养微生物的主要原因,介绍了当前出现的已经证实卓有成效的新型培养基及培养方法,旨在为研究开发更多创新的培养方法提供参考借鉴。

关键词: 难培养微生物, 培养方法, 新药物, 微观世界

Abstract: The culture-independent technique of 16S rRNA clone and sequencing in samples of environments suggests that there exists a host of unique and culture-independent microbes in nature. It is an important subject to learn how to explore as many as microbes. In view of that, this paper reviews the mainly reasons why is difficult for us to culture these culture-independent microbes and introduces the innovative culture technique and the improved culture medium those are proved stunning effective recently. By doing that we believe more and more innovative culture technique will show up in near future.

Key words: culture-independent microbes, culture technique, new drug, microcosm

曾建民,曾振顺,原红娟,谭志远. 难培养微生物培养方法的研究进展[J]. 生物技术进展, 2012, 2(3): 165-170.

ZENG Jian-min, ZENG Zhen-shun, YUAN Hong-juan, TAN Zhi-yuan*. Advances in Studies of Culture Methods of Oligotrophic Microbes[J]. journal1, 2012, 2(3): 165-170.

难培养微生物培养方法的研究进展

Advances in Studies of Culture Methods of Oligotrophic Microbes

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