基于CiteSpace分析核酸适体（Aptamer）技术研究现状

doi:10.19586/j.2095-2341.2021.0127

生物技术进展 ›› 2021, Vol. 11 ›› Issue (6): 783-794.DOI: 10.19586/j.2095-2341.2021.0127

基于CiteSpace分析核酸适体（Aptamer）技术研究现状

梁永羿¹(), 龙粤², 冼嘉华¹, 林骏¹, 彭进²()

^1.深圳市药品检验研究院（深圳市医疗器械检测中心），广东深圳 518057
^2.凯杰生物工程（深圳）有限公司，广东深圳 518000

收稿日期:2021-07-02 接受日期:2021-09-30 出版日期:2021-11-25 发布日期:2021-11-26
通讯作者: 彭进
作者简介:梁永弈，E-mail： ksgt00533994@163.com
基金资助:
广东省药品监督管理局2020年科技创新项目(2020ZDB10)

Analysis on the Research Status of Aptamer Technology Based on CiteSpace

Yongyi LIANG¹(), Yue LONG², Jiahua XIAN¹, Jun LIN¹, Jin PENG²()

^1.Shenzhen Institute for Drug Control （Shenzhen Testing Center of Medical Devices），Guangdong Shenzhen 518057，China
^2.QIAGEN Bioengineering （Shenzhen） Co. ，Ltd，Guangdong Shenzhen 518000，China

Received:2021-07-02 Accepted:2021-09-30 Online:2021-11-25 Published:2021-11-26
Contact: Jin PENG

摘要/Abstract

摘要：

利用可视化分析软件，客观、全面地展示和分析适体的研究现状、研究热点和发展趋势，为适体的研究提供指导和参考。以SCI数据库Web of Science平台为数据源，采用CiteSpace 5.5.R2软件对1900年1月1日至2020年12月31日的适体相关文献进行分析。共纳入文献14 939篇。在该领域发表论文最多的作者是WEIHONG TAN、KHALIL ABNOUS等；近年来突现的主题词是“wide linear range”“pg ml”“clinical diagnosis”“early diagnosis”等，是目前该领域的研究热点；作者共被引分析和文献共被引分析表明，该领域的研究主要集中在适体的体外筛选、适体构象的变化、适体在药物靶向治疗中的应用及适体生物传感器。结果表明，利用CiteSpace软件对Web of Science数据库中已发表的适体文献进行分析，揭示了该领域的研究热点从体外筛选和适体构象变化（1992—2002）到适体在药物靶向治疗和适体生物传感器中的应用（2002—2020）。结合近年来突现的主题词预测适体在药物靶向治疗和适体生物传感器中的应用仍将是未来的研究热点。

关键词: Citespace, 适体, 文献计量学, 可视化分析

Abstract:

To objectively and comprehensively display and analyze the research status， research hotspots and development trend of aptamer by using visual analysis software， so as to provide guidance and reference for the research of aptamer. Using the Web of Science platform database as the data source， CiteSpace 5.5.R2 software was used to analyze the literature related to aptamer from January 1st， 1900 to December 31th， 2020. A total of 14 939 articles were included. WEIHONG TAN， KHALIL ABNOUS and other authors published the most papers in this field. In recent years， the emerging terms were “wide linear range”“pg ml”“clinical diagnosis”“early diagnosis”， etc， these terms are the research hotspots in this field. The author co?citation analysis and literature co?citation analysis showed that the research in this field mainly focused on the in vitro screening of aptamers， the change of aptamer conformation， the application of aptamers in drug targeted therapy and aptamer biosensors. CiteSpace software was used to analyze the published aptamer literatures in Web of Science database， revealing the research hotspots in this field， from in vitro screening and aptamer conformation change （1992—2002） to the application of aptamers in drug targeted therapy and aptamer biosensor （2002—2020）. Combined with the emerging terms in recent years， it is predicted that the application of aptamer in drug targeted therapy and aptamer biosensor will continue to be the future research direction.

Key words: CiteSpace, aptamer, bibliometrics, visual analysis

中图分类号:

S951.4+2

梁永羿, 龙粤, 冼嘉华, 林骏, 彭进. 基于CiteSpace分析核酸适体（Aptamer）技术研究现状[J]. 生物技术进展, 2021, 11(6): 783-794.

Yongyi LIANG, Yue LONG, Jiahua XIAN, Jun LIN, Jin PENG. Analysis on the Research Status of Aptamer Technology Based on CiteSpace[J]. Current Biotechnology, 2021, 11(6): 783-794.

图/表 13

参考文献 66

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排名	发文作者	国家	发文量/篇	发文起始年份	占总文献比例/%
1	WEIHONG TAN	美国	241	2006	1.613
2	KHALIL ABNOUS	伊朗	125	2015	0.837
3	KEMIN WANG	中国	119	2008	0.797
4	MOHAMMAD RAMEZANI	伊朗	116	2015	0.776
5	RUO YUAN	中国	115	2010	0.770
6	SEYED MOHAMMAD TAGHDISI	伊朗	107	2015	0.716
7	ZHOUPING WANG	中国	95	2013	0.636
8	NUO DUAN	中国	72	2013	0.482
9	YAQIN CHAI	中国	72	2010	0.482
10	SHIJIA WU	中国	68	2013	0.455

排名	发文作者	国家	发文量/篇	发文起始年份	占总文献比例/%
1	WEIHONG TAN	美国	241	2006	1.613
2	KHALIL ABNOUS	伊朗	125	2015	0.837
3	KEMIN WANG	中国	119	2008	0.797
4	MOHAMMAD RAMEZANI	伊朗	116	2015	0.776
5	RUO YUAN	中国	115	2010	0.770
6	SEYED MOHAMMAD TAGHDISI	伊朗	107	2015	0.716
7	ZHOUPING WANG	中国	95	2013	0.636
8	NUO DUAN	中国	72	2013	0.482
9	YAQIN CHAI	中国	72	2010	0.482
10	SHIJIA WU	中国	68	2013	0.455

聚类 ID	大小	轮廓值	平均引用年限	代表作者	研究内容
0	40	0.991	2010	WEIHONG TAN	纳米材料与适体结合用于肿瘤细胞的鉴别、分析
1	28	0.972	2015	KEMIN WANG	适体用于生物分子的检测
2	19	0.979	2013	ZHILIANG JIANG，KUN WANG	适体结合共振散射光谱检测靶物质
3	19	0.998	2015	MOHAMMAD RAMEZANI，ZHOUPING WANG等	适体用于药物的靶向治疗及生物传感器的研究

聚类 ID	大小	轮廓值	平均引用年限	代表作者	研究内容
0	40	0.991	2010	WEIHONG TAN	纳米材料与适体结合用于肿瘤细胞的鉴别、分析
1	28	0.972	2015	KEMIN WANG	适体用于生物分子的检测
2	19	0.979	2013	ZHILIANG JIANG，KUN WANG	适体结合共振散射光谱检测靶物质
3	19	0.998	2015	MOHAMMAD RAMEZANI，ZHOUPING WANG等	适体用于药物的靶向治疗及生物传感器的研究

频率	主题词	最早出现年	聚类	突现值	主题词	最早出现年	聚类	中心性	主题词	最早出现年	聚类
2 398	Detection limits	2002	1	121.75	Vitro selections	1992	0	0.21	Conformational change	1994	5
1 122	DNA aptamers	1994	0	89.99	RNA aptamers	1996	0	0.13	DNA aptamers	1994	0
961	Gold nanoparticles	2006	1	51.98	Secondary structure	1994	0	0.12	High affinities	1994	0
958	High selectivity	2004	1	42.82	Pg ml	2018	1	0.11	Inhibitory effects	1996	6
953	High affinities	1994	0	42.78	Wide linear range	2018	1	0.09	High specificity	2000	1

基于CiteSpace分析核酸适体（Aptamer）技术研究现状

Analysis on the Research Status of Aptamer Technology Based on CiteSpace

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参考文献 66

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聚类 ID	大小	轮廓值	平均引用年限	主要主题词
0	90	0.612	1999	High affinities, SELEX, vitro selections, target molecules, secondary structure
1	69	0.784	2008	Detection limits, gold nanoparticles, high selectivity, sensitive detection, high sensitivity, high specificity, wide linear range, early diagnosis
2	54	0.784	1996	Complex formation, solution structure, RNA structure, structural motifs, structural basis
3	37	0.824	1997	Human thrombin, circular dichroism, vascular endothelial growth factor
4	32	0.735	1998	Model system, quadruplex structure, three‑dimensional structure, specific inhibitors
5	27	0.888	1997	Conformational change, single‑stranded DNA, tertiary structure, double‑stranded DNA, allosteric ribozymes, catalytic activity
6	10	0.998	1996	Inhibitory effects, antiproliferative effects, systematic approach

聚类 ID	大小	轮廓值	平均引用年限	主要被引作者
0	59	0.871	2011	LIU J W, WANG J, WANG Y, ZHANG Y, LI H, LI J
1	58	0.841	2004	ELLINGTON A D, TUERK C, BOCK L C, ZHOU J H, TOMBELLI S, KIM Y S
2	49	0.911	1998	HUIZENGA D E, FAMULOK M, WILSON D S, JENISON R D
3	27	0.967	1994	MACAYA R F, WANG K Y, PADMANABHAN K
4	18	1.000	1993	KARN J, HEAPHY S, SULLENGER B A
5	13	0.938	1995	ROBERTSON D L, MILLIGAN J F, PUGLISI J D, HEUS H A
6	10	0.991	1993	PABORSKY L R, WU Q Y, GRIFFIN L C

被引频率	作者	最早发文年	聚类	突现值	作者	最早发文年	聚类	中心性	作者	最早发文年	聚类
3 561	ELLINGTON A D	1992	1	124.68	ZHOU J H	2018	1	0.30	WANG Y	1993	0
3 484	TUERK C	1992	1	120.13	ZHANG Y	2016	0	0.29	ELLINGTON A D	1992	1
1 244	LIU J W	2006	0	111.25	LI H	2016	0	0.26	TUERK C	1992	1
1 168	WANG J	2006	0	104.05	LI J	2015	0	0.18	BOCK L C	1993	1
1 101	BOCK L C	1993	1	102.04	PAVLOV V	2006	0	0.12	HUIZENGA D E	1996	2
1 055	WANG Y	1993	0	99.75	LI X	2016	0	0.12	PABORSKY L R	1994	6

聚类 ID	大小	轮廓值	平均引用年限	主要研究内容	主要文献
0	56	0.867	1994	体外筛选适体技术研究	[29⁃32]
1	42	0.822	2006	适体在生物传感器方面的研究	[33⁃36]
2	40	0.785	2010	适体在诊断治疗上的应用研究	[37⁃44]
3	38	0.887	2015	适体在药物靶向传送方面的研究	[45⁃46]
4	27	0.885	2000	开发基于适体的通用荧光传感器	[47⁃48]
5	23	0.821	1997	开发特异性识别细胞的适体	[49⁃51]
6	23	0.979	1991	开发基于适体的凝血酶抑制剂	[52⁃53]
7	6	0.988	2003	适体在参与调节生物体代谢过程的应用	[54⁃55]

共被引频率	作者（国家）	聚类	发表年份	主要内容	文献
504	KEEFE A D（美国）	2	2010	适体药物应用方面进展	[39]
438	LIU J W（美国）	2	2009	适体在生物传感器方面的进展	[40]
292	SHANGGUAN D（美国）	2	2006	筛选特异性识别白血病细胞的适体	[37]
279	WILLNER I（以色列）	1	2010	电子适体传感器应用进展	[33]
260	CHO E J（美国）	2	2009	电子适体传感器应用进展	[41]
253	LIU J W（美国）	1	2005	适体与纳米金粒子结合用于生物传感器，靶标与适体结合造成连接在适体上的纳米金释放出来，从而产生颜色变化；可作为一种通用的传感器设计	[34]
252	FANG X H（中国）	2	2010	适体用于特异性识别肿瘤细胞	[42]
248	SONG S P（中国）	1	2008	适体在生物传感器方面的进展	[35]
247	ILIUK A B（美国）	2	2011	适体在生物分析中的应用进展	[43]
247	TAN W H（中国/美国）	2	2013	基于细胞选择的适体在生物分析中的应用进展	[38]
247	FAMULOK M（德国）	2	2007	功能适体和适体酶在诊断和治疗上的应用进展	[44]

中心性	作者（国家）	聚类	发表年份	主要内容	文献
0.55	NUTIU R（加拿大）	1	2003	设计了一种基于适体的荧光检测系统，含荧光基团的单链核酸与含淬灭基团的单链核酸在没有靶标存在时均与适体互补形成双链，荧光基团与含淬灭基团接近无法发出荧光，当存在靶标时，适体与靶标结合，含淬灭基团的单链核酸被释放，从而发出荧光	[36]
0.41	WILSON D S（美国）	0	1999	功能性核酸体外筛选方法的进展	[29]
0.34	HUIZENGA D E（美国）	0	1995	利用体外筛选的方法，分离出结合腺苷/ATP的适体；并提出了一个ATP结合DNA的模型结构	[30]
0.29	LIU J W（美国）	2	2009	适体在生物传感器方面的应用进展	[40]
0.29	BOCK L C（美国）	6	1992	筛选特异结合并抑制凝血酶活性的单链DNA适体	[52]
0.18	JHAVERI S（美国）	4	2000	建立通用的筛选有信号转导功能的适体方法（有荧光标记，且适体与靶标结合构象发生变化时荧光信号强度会发生变化）；在适体筛选的序列中引入少量荧光标记的尿苷，获得的腺苷适体在腺苷同源物ATP存在下，其荧光会增强，通过观察荧光强度变化即可确认靶标物质的存在	[47]
0.17	LIU J W（美国）	1	2005	适体与纳米金粒子结合用于生物传感器，靶标与适体结合造成连接在适体上的纳米金释放出来，从而产生颜色变化；可作为一种通用的传感器设计	[34]
0.15	GOLD L（美国）	5	1995	SELEX技术进展	[50]
0.14	ILIUK A B（美国）	2	2011	适体在生物分析中的应用进展	[43]
0.13	SHANGGUAN D（美国）	2	2006	特异性识别白血病细胞的适体	[37]
0.12	DANIELS D A（美国）	5	2003	利用SELEX技术筛选特异性识别肿瘤细胞的适体	[49]
0.11	STOJANOVIC M N （美国）	4	2001	开发基于适体的可卡因荧光传感器。适体5′端和3′端分别有荧光基团和淬灭基团，无靶标时，荧光基团和淬灭基团分离，可以发出荧光；当可卡因存在时，适体结合可卡因并发生构象变化，荧光基团和淬灭基团靠近，荧光被淬灭	[48]
0.11	SASSANFAR M（美国）	0	1993	开发了一种用于体外筛选ATP适体的新方法	[31]
0.11	MORRIS K N（美国）	5	1998	利用SELEX技术筛选特异性识别人红细胞膜的适体	[51]
0.10	WILLNER I（以色列）	1	2010	电子适体传感器应用进展	[33]
0.10	DENG B（加拿大）	3	2014	蛋白质适体研究进展	[45]
0.10	JIANG F（美国）	0	1996	研究RNA适体的空间结构	[32]

突现值	作者（国家）	聚类	发表年份	主要内容	文献
144.29	KEEFE A D（美国）	2	2010	适体药物应用方面的进展	[39]
107.59	ZHOU J H（美国）	3	2017	适体作为药物靶向传送剂在临床转化上存在的挑战	[46]
103.52	LIU J W（美国）	2	2009	适体在生物传感器方面的应用	[40]
87.29	ILIUK A B（美国）	2	2011	适体在生物分析中的应用	[43]
81.23	SHANGGUAN D（美国）	2	2006	特异性识别白血病细胞的适体	[37]

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