关键词: CRISPR-Cas13系统, HEPN结构域, RNA干扰, Type VI-A/B/D

Abstract: CRISPR-Cas13 system is a fast, efficient and accurate RNA editing tool, which has characters of easy to design, simple structure, convenient operation and strong specificity. This paper summarized the position of CRISPR-Cas13 in CRISPR classification system, the structural basis and mechanism of CRISPR-Cas13, the comparison with other RNA level adjustment methods and the current application prospect, which was expected to provide reference for related research.

Key words: CRISPR-Cas13 system, HEPN domain, RNA interference, Type VI-A/B/D