关键词: LPEI, 脂质体, 慢病毒包装, 白血病细胞系

Abstract: Gene overexpression and knock down mediated by lentivirus is often used in leukemia research. There are some problems, such as high cost and low efficiency in suspension cell infection. In order to identify the effectiveness and safety of the lentivirus packaged with different transfection method in leukemia research, solving the problems of low infection efficiency and high cost during the lentivirus production, we packaged lentiviruses with linear polyethylenimine (LPEI) and liposomes, and then compared the infection ability and the effects on biological characteristics of leukemia cell lines of lentiviruses produced with these two methods. Using LPEI or liposomes to generate lentiviral particles, respectively, our studies showed that the titer of lentivirus packaged with LPEI was slightly higher than that packaged with liposomes under the same packaging conditions. Three leukemia cell lines (K562, HL60 and HEL) were infected with the same amount lentiviral particles according to multiplicity of cellular infection (MOI) and lentiviral titer, and there was no difference in infection efficiency between the two groups. The cell proliferation of leukemic cells infected with lentivirus produced with LPEI was slightly slower than that in liposome group, the effects on induced differentiation and colony forming ability of these two methods were similar. Therefore, using lentivirus produced with LPEI transfection method can be an economical and practical choice in experimental studies in leukemia.

Key words: LPEI, liposomes, lentiviral packaging, leukemia cell lines