关键词: 蓝光受体, 蛋白降解, 开花时间, 下胚轴, PRP8

Abstract: Plant perceive environment light through photoreceptors to regulate self-growth and development. Cryptochrome 1（CRY1）and Cryptochrome 2（CRY2）are specific blue light photoreceptors in Arabidopsis. CRY2 undergoes blue-light dependent phosphorylation and degradation process, but the mechanism underlie this process is not clear. An early flower mutant was obtained through screening the EMS mutagenesis pools of LUC-CRY2/Col-4 transgene lines. CRY2 protein was over-accumulated in the mutant but the amount of CRY1 protein was not affected. This mutant was named mac1 (more abundant of cry2 1). Map-based cloning and CRISPR/Cas9 knock out experiment showed that this is a new allele of mutation in PRP8, we named it prp8-11. Real-time PCR demonstrated that the accumulation of CRY2 protein is not caused by overexpression of mRNA, but regulated at post transcription level. This study provided new insight for further understanding the function of the PRP8 gene in light signal network.

Key words: cryptochrome, protein degradation, flowering time, hypocotyls, PRP8