蒺藜苜蓿MtFVE基因功能初步解析

doi:10.19586/j.2095-2341.2019.0014

生物技术进展 ›› 2019, Vol. 9 ›› Issue (2): 152-160.DOI: 10.19586/j.2095-2341.2019.0014

蒺藜苜蓿MtFVE基因功能初步解析

王瑞良1,张鹏程2,牛丽芳2,朱昊3,李学森3,王兴春1*,林浩2*

1.山西农业大学生命科学学院, 山西太谷 030801;

2.中国农业科学院生物技术研究所, 北京 100081;

3.新疆畜牧科学院草业研究所, 乌鲁木齐 830000

收稿日期:2019-02-07 出版日期:2019-03-25 发布日期:2019-02-22
通讯作者: 王兴春，教授，主要从事植物抽穗开花调控机制的研究。E-mail：wxingchun@163.com；林浩，研究员，主要从事苜蓿功能基因组学研究。E-mail：linhao@caas.cn
作者简介:王瑞良，硕士研究生，研究方向为植物生物化学与分子生物学。E-mail：wangruiliang009@163.com。
基金资助:
中国农业科学院农业科技创新工程重大产出科研选题项目（CAAS-ZDXT2019004）；自治区重点研发计划项目（2018B01006-3）资助。

Preliminary Function Analysis of the MtFVE gene in Medicago truncatula

WANG Ruiliang, ZHANG Pengcheng, NIU Lifang, ZHU Hao, LI Xuesen, WANG Xingchun, LIN Hao

1.College of Life Sciences, Shanxi Agricultural University, Shanxi Taigu 030801, China;

2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;

3.Grassland Research Institute, Xinjiang Academy of Animal Sciences, Urumqi 830000, China

Received:2019-02-07 Online:2019-03-25 Published:2019-02-22

摘要/Abstract

摘要： 拟南芥（Arabidopsis thaliana）中自主通路成员FLOWERING LOCUS VE（FVE）主要通过表观遗传学机制抑制其下游开花抑制子FLOWERING LOCUS C（FLC）的表达从而促进开花，但有研究发现蒺藜苜蓿（Medicago truncatula）中没有FLC的同源基因，因此，蒺藜苜蓿中FVE同源基因调控开花时间的分子作用机理还有待研究。通过生物信息学方法在蒺藜苜蓿中筛选到2个拟南芥FVE的同源蛋白，将其分别命名为MtFVEa和MtFVEb。经氨基酸序列比对发现，2个蛋白均具有典型的WD40蛋白结合结构域。随后通过qRT-PCR和半定量RT-PCR检测MtFVEa和MtFVEb基因组织表达特异性，发现2个基因在花和营养生长期的茎尖具有较高水平的表达。经酵母双杂实验进一步分析发现，MtFVEa蛋白与MtFLD \[拟南芥FLOWERING LOCUS D（FLD）的同源蛋白\] 和MtHDA6 \[拟南芥histone deacetylase 6（HDA6）的同源蛋白\] 均发生蛋白互作，表明MtFVE可能同样形成FVE-FLD-HDA6三元复合体参与蒺藜苜蓿开花调控。研究结果为进一步探索蒺藜苜蓿MtFVE基因的功能提供了研究思路和理论支持。

关键词: 蒺藜苜蓿, MtFVE, FVE-FLD-HDA6

Abstract: FLOWERING LOCUS VE (FVE), one of the autonomous pathway members in Arabidopsis thaliana, promotes the floral transition by inhibiting transcriptional expression of FLOWERING LOCUS C (FLC) at the chromatin level. FLC acts as a key repressor of oral transition by blocking the expression of integrator genes. Howerver, Medicago truncatula lacks the FLC gene. Therefore, the molecular mechanism underlying the regulation of flowering time by FVE homolog in M. truncatula remains to be elucidated. There were two FVE homologs in Medicago truncatula, named MtFVEa and MtFVEb, respectively, and they both had a typical WD40 protein binding domain. Subsequently, qRT-PCR and semi-quantitative RT-PCR analysis revealed that both MtFVEa and MtFVEb were highly expressed in flower and shoot apices. Yeast double-hybrid assay showed that MtFVEa interacted with MtFLD (FLOWERING LOCUS D, a homologous protein of Arabidopsis FLD) and MtHDA6 (histone deacetylase 6, a homologous protein of Arabidopsis HDA6), suggested that MtFVE may be involved in flowering time regulation by forming the FVE-FLD-HDA6 complex in M. truncatula. The research provided ideas and theory for further research on the function of MtFVE in M. truncatula.

Key words: M. truncatula, MtFVE, FVE-FLD-HDA6

王瑞良,张鹏程,牛丽芳,朱昊,李学森,王兴春,林浩. 蒺藜苜蓿MtFVE基因功能初步解析[J]. 生物技术进展, 2019, 9(2): 152-160.

WANG Ruiliang1, ZHANG Pengcheng2, NIU Lifang2, ZHU Hao3, LI Xuesen3, WANG Xingchun1*, LIN Hao2*. Preliminary Function Analysis of the MtFVE gene in Medicago truncatula[J]. Curr. Biotech., 2019, 9(2): 152-160.

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蒺藜苜蓿MtFVE基因功能初步解析

Preliminary Function Analysis of the MtFVE gene in Medicago truncatula

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