关键词: 小胶质细胞, 模式识别受体, 磷酸化, 吞噬

Abstract: To investigate the effect of pattern recognition receptor(PRR) activation state on Aβ42-induced p38-MAPK phosphorylation and phagocytosis ability in microglia, normal microglia and PRR（SRA，TRL2，SRB，CD36）-blocked microglia was incubated with oligomer form of Aβ42（oAβ42）. The level of p38-MAPK phosphorylation and the effect of the blocking of PRR in microglia on cell uptake of Aβ42 was measured. Results showed that, antibody-blocking SRA and TLR2 receptor reduced the p38-MAPK phosphorylation level induced by Aβ42 significantly, compared with normal microglia. Antibody-blocking toll-like receptor 2(TRL2) neutralization lower the cell uptake of Aβ42, which indicated that the PRR activation state had influenced the phagocytosis ability of microglia, and the process was related with the p38-MAPK phosphorylation level. However，endocytosis inhibiter-treated microglia had no significant influence on oAβ42 uptake amount, which indicated that endocytosis on Aβ42 was not classical endocytosis. Toll-like receptor 2（TLR2）was involved in oAβ42 phagocytosis initiation and would be potential therapeutic target for AD immunotherapy.

Key words: microglia, pattern recognition receptor, phosphorylation, phagocytosis