关键词: 细菌多样性, 菠菜, 生菜, 土壤细菌

Abstract: To study the effect of lettuce and spinach planting on soil cultivable bacteria diversity, and in order to understand the changes of soil bacteria after planting of these two kinds of vegetables, using R2A media, we isolated bacterial strains, and phylogeneticly analized comparison system and preliminary identification of these strains based on 16S rRNA gene sequence. Soil samples grown lettuce were isolated 109 strains of bacteria, mainly belonged to 4 phyla, 19 families, 23 genera. Soil samples grown spinach were isolated 126 strains of bacteria, mainly belonged to 3 phyla, 21 genera and 16 families. In phyla, the dominant bacteria in the soil of planting lettuce were Firmicutes (39.4%), Actinobacteria (33.9%) and Proteobacteria (24.8%); the dominant bacteria in the soil sample of planting spinach were Actinobacteria (64.3%), Firmicutes (27%), and Proteobacteria (8.7%). At the genus level, the difference between the two types of soil samples was greater.

Key words: bacterial diversity, spinach, lettuce, soil bacteria