关键词: 氮同化, 硝酸还原酶, 亚硝酸还原酶, 转录后修饰, 翻译后调控

Abstract: Plants take up nitrogen in the form of nitrate and ammonia through nitrate assimilation pathway. Nitrate assimilation is highly regulated process by two successive enzymes, nitrate reductase (NR) and nitrite reductase (NiR), which mainly controll the primary nitrogen assimilation. In higher plants, various external and endogenous factors influence the regulation of NR and NiR gene expression transcriptionally as well as post-transcriptionally. Post-translational modulation is an important mechanism to eliminate the accumulation of nitrite. Development of molecular biology methods will contribute to the research of regulatory networks of NR and NiR by creating mutants and transgenic lines.

Key words: nitrogen assimilation, nitrate reductase, nitrite reductase, post-transcription, post-translational regulation