关键词: 大肠杆菌表达系统, 酵母表达系统, 表达载体, 宿主菌改造

Abstract: Since the 70s of 20th century, the rapid development of molecular biology and genomics have played increasingly important role in the field of biological and medical science. In fermentation industry，molecular biology techniques are widely used in construction of genetically engineered bacteria and strains improvement, in order to improve the yield of fermentation products and enrich fermentation product types. In the past decade, the use of prokaryotic and eukaryotic expression systems for exogenous gene amplification and expression to produce proteins vaccines, DNA vaccines and enzymic preparations, etc. is the emerging field of fermentation industry. In this paper, we reviewed the recent progress on E. coli expression systems and yeast expression systems from two aspects of vectors and host transformation.

Key words: Escherichia coli expression system, Yeast expression system, expression vectors, host bacteria transformation