生物技术进展 ›› 2014, Vol. 4 ›› Issue (5): 318-324.DOI: 10.3969/j.issn.2095-2341.2014.05.03

• 进展评述 • 上一篇    下一篇

植物油体在生物技术中的应用研究进展

姚正颖,张卫明,孙力军*   

  1. 南京野生植物综合利用研究院, 南京 210042
  • 收稿日期:2014-09-01 出版日期:2014-09-25 发布日期:2014-09-11
  • 通讯作者: 孙力军,博士,副研究员,主要从事植物资源综合利用研究。E-mail:shengwudiqiu@126.com
  • 作者简介:姚正颖,博士,助理研究员,主要从事植物生理与资源利用研究。E-mail:belinda227@163.com。
  • 基金资助:

    国家自然科学基金项目(31100224);江苏省自然科学基金项目(BK2011131)资助。

Research Progress in Biotechnological Applications of Oil Bodies

YAO Zheng-ying, ZHANG Wei-ming, SUN Li-jun   

  1. Nanjing Institute for the Comprehensive Utilization of Wild Plant, Nanjing 210042, China
  • Received:2014-09-01 Online:2014-09-25 Published:2014-09-11

摘要: 油体是植物种子尤其是油料植物种子的重要贮脂细胞器,具有较强的物化稳定性,而且易于通过离心法分离提取。研究表明,油体是由外层的磷脂和油体结合蛋白以及包裹在内部的液态基质(主要为三酰甘油)形成的弹性球体或椭球体。目前,在植物中共发现三类油体结合蛋白,它们主要存在于油体表面。鉴于油体和油体结合蛋白的结构特殊性,二者在生物技术领域得到了广泛应用。本文重点综述了油体在表达纯化外源蛋白方面的优势、策略以及在生产药用蛋白、制备固定化酶、捕捉抗体和药用酶、生产营养素和提高植物抗性等多个领域的研究进展,并介绍了人工油体和油体乳化剂方面的开发应用情况。

关键词: β-甘露聚糖酶, 嗜热酶, 基因资源, 分子改良

Abstract: Oil bodies are the important oil storage organelles in plant seeds, particularly in oil plant seeds. Oil bodies have strong physical and chemical stability and are easily separated by centrifugation. Studies showed that oil bodies have a spherical or ellipsoid shape and are composed of outer phospholipid, integral proteins and inner liquid matrix (mostly triacylglycerol). To date,three types of integral proteins have been found in plants and are mainly localized on the surface of oil bodies. In view of the unique structures of oil bodies and integral proteins,both of them have been widely applied in the field of biotechnology. This review summarized advantages and strategies of using oil bodies in expression and purification of recombinant proteins,as well as its applications in the fields of production of pharmaceutical protein,preparation of immobilized enzymes,affinity capture of antibodies and pharmaceutical enzymes,production of nutraceuticals,enhancement of plant resistance against pathogen. The development and applications of artificial oil bodies and oil-body-emulsifying-agents were also discussed.

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