关键词: 基因拆分, 内含肽, 基因飘流

Abstract: Genetically modified (GM) safety problems caused increasingly debate as the planting area of GM crops improving year by year, in which, gene flow from transgenic crops to wild relatives by pollen spread is one of the focus of attention. Gene split technology provides a new way to limit transgenic wander from GM crops. It is based on the intein-mediated protein splicing. It has been proved that different gene was split and the expression products were re-assembled into functional protein in arabidopsis, tobacco, wheat and other crops by this method until now. These successful cases provide theoretical basis and experimental support for using this method to limit gene flow of GM. In this article， we reviewed the research progress on theoretical basis of gene split and the application of gene split technology on gene flow control.

Key words: gene split, intein, gene flow