基于菌丝生物量和胞外多糖产量的Simplicillium lanosoniveum液体发酵培养条件优化

doi:10.19586/j.2095-2341.2024.0090

生物技术进展 ›› 2024, Vol. 14 ›› Issue (6): 937-946.DOI: 10.19586/j.2095-2341.2024.0090

• 食药用菌生物技术专题 • 上一篇下一篇

基于菌丝生物量和胞外多糖产量的Simplicillium lanosoniveum液体发酵培养条件优化

李昕阳¹(), 杨阳²^,³(), 刘栋⁴^,⁵, 刘宇光¹, 刘俊泽¹, 陈长宝¹, 王欢¹^,³(), 王淑敏¹()

^1.长春中医药大学，吉林省人参科学研究院，长春 130117
^2.中国热带农业科学院环境与植物保护研究所，海口 572829
^3.吉林农业大学教育部食用药用真菌工程研究中心，长春 130022
^4.中南林业科技大学生命与环境学院，湖南省林业生物技术重点实验室，森林资源生物技术科技创新国际合作基地，长沙 410004
^5.韩国顺天国立大学，韩国地衣研究所，韩国顺天 57922

收稿日期:2024-04-24 接受日期:2024-07-30 出版日期:2024-11-25 发布日期:2024-12-27
通讯作者: 王欢,王淑敏
作者简介:李昕阳E-mail: 17634093266@163.com
杨阳 E-mail: yyjob1992@163.com第一联系人：李昕阳和杨阳为共同第一作者。
基金资助:
吉林省中医药科技项目(2023009);吉林省科技发展计划项目(YDZJ202301ZYTS509);吉林省科技发展计划项目(20240303118NC)

Optimization of Liquid Fermentation Conditions for Mycelial Biomass and Exopolysaccharide Production of Simplicillium lanosoniveum

Xinyang LI¹(), Yang YANG²^,³(), Dong LIU⁴^,⁵, Yuguang LIU¹, Junze LIU¹, Changbao CHEN¹, Huan WANG¹^,³(), Shumin WANG¹()

^1.Jilin Ginseng Academy，Changchun University of Chinese Medicine，Changchun 130117，China
^2.Environment and Plant Protection Institute，Chinese Academy of Tropical Agricultural Sciences，Haikou 572829，China
^3.Engineering Research Center of Edible and Medicinal Fungi，Ministry of Education，Jilin Agricultural University，Changchun 130022，China
^4.Hunan Provincial Key Laboratory for Forestry Biotechnology，International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology，College of Life and Environmental Sciences，Central South University of Forestry and Technology，Changsha 410004，China
^5.Korea Lichen Research Institute，Sunchon National University，Soonchun 57922，Korea

Received:2024-04-24 Accepted:2024-07-30 Online:2024-11-25 Published:2024-12-27
Contact: Huan WANG,Shumin WANG

摘要/Abstract

摘要：

真菌Simplicillium lanosoniveum已被报道具有多种生物学效应，往往被用作生物防治。然而，有关其液体发酵的研究却很少。通过形态学和系统发育分析，鉴定了1株从发网菌属中分离出的丝孢真菌SL001，即S. lanosoniveum。以菌丝生物量和胞外多糖产量为指标，通过单因素和正交实验优化发酵培养基的组成和比例，以阐明液体发酵S. lanosoniveum的最优培养条件。结果表明，在麦芽糖40 g·L^-1、蛋白胨3 g·L^-1和KH₂PO₄ 1 g·L^-1的营养条件下，S. lanosoniveum的菌丝生物量和胞外多糖产量最高，对S. lanosoniveum菌丝生物量的影响程度由大到小依次为麦芽糖>蛋白胨>KH₂PO₄。最后，根据营养需求对培养条件进一步优化。结果显示，最佳培养条件为麦芽糖40 g·L^-1，蛋白胨3 g·L^-1，KH₂PO₄ 1 g·L^-1，培养温度28 ℃，pH 5.5，转速150 r·min^-1，装液量80 mL/250 mL，接种量6%。在最佳培养条件下，菌丝生物量和胞外多糖可达到最大值1.69 g·100 mL^-1和3.968 mg·ml^-1，与对照组相比有显著差异（P<0.01）。通过单因素和正交实验得到真菌S. lanosoniveum的最佳液体发酵条件，为进一步研究S. lanosoniveum的抗菌作用和工业化生产提供了理论依据。

关键词: S. lanosoniveum, 液体发酵，营养成分, 培养条件, 菌丝生物量, 胞外多糖含量

Abstract:

Simplicillium lanosoniveum has been reported to have a variety of biological effects， and has been traditionally used as a biological control agent. However， there were few reports on its liquid fermentation. In this study， a hyphomycetous fungus SL001 isolated from Stemonitis splendens was identified as S. lanosoniveum through morphology and phylogenetic analysis. Then， taking mycelial biomass and exopolysaccharides yield as indicators， the composition and proportion of fermentation medium were optimized through single-factor and orthogonal experiments to clarify the nutritional needs of liquid fermentation.The results showed that the highest mycelial biomass and exopolysaccharides yield were harvested under the nutritional conditions of 40 g·L^-1 maltose， 3 g·L^-1 peptone and 1 g·L^-1 KH₂PO₄， and the influence on mycelial biomass of S. lanosoniveum was maltose>peptone>KH₂PO₄. Finally， the culture conditions were further optimized based on nutritional needs. The results showed that the optimum liquid fermentation conditions were 28 ℃， pH 5.5， 150 r·min^-1， 80 mL/250 mL and 6% inoculum size. The mycelial biomass and exopolysaccharides yield were as high as 1.69 g·100 mL^-1 and 3.968 mg·mL^-1 respectively， which were significantly different from the control （P<0.01）. This work provides a theoretical basis for further research on antibacterial agent function and industrial production of S. lanosoniveum.

Key words: S. lanosoniveum, liquid fermentation, nutritional factors, culture conditions, mycelial biomass, exopolysaccharide production

中图分类号:

Q914.83

李昕阳, 杨阳, 刘栋, 刘宇光, 刘俊泽, 陈长宝, 王欢, 王淑敏. 基于菌丝生物量和胞外多糖产量的Simplicillium lanosoniveum液体发酵培养条件优化[J]. 生物技术进展, 2024, 14(6): 937-946.

Xinyang LI, Yang YANG, Dong LIU, Yuguang LIU, Junze LIU, Changbao CHEN, Huan WANG, Shumin WANG. Optimization of Liquid Fermentation Conditions for Mycelial Biomass and Exopolysaccharide Production of Simplicillium lanosoniveum[J]. Current Biotechnology, 2024, 14(6): 937-946.

图/表 12

表1 本研究中用于系统发育分析的序列信息

Table 1 Sequences information used for phylogenetic analysis in this study

物种名	菌株名	登录号	国家/地区	分离来源
Cordyceps pseudomilitaris	NHJ6	AJ786589	-	-
Lecanicillium lecanii	CBS 101247	AJ292382	西印度群岛	Coccus viridis
Simplicillium aogashimaense	JCM 18167	AB604002	日本伊豆岛	Asplenium antiquum下的土壤
S. calcicola	LC5371	KU746705	中国贵州省	岩石
S. calcicola	LC5586	KU746706	中国贵州省	岩石
S. chinense	LC1345	JQ410324	中国	木材
S. cylindrosporum	JCM 18169	AB603989	日本小笠原岛	Cinnamomumpseudopedunculatum下的土壤
S. lamellicola	CBS 116.25	MH854806	英国	-
S. lamellicola	CBS:596.71	MH860281	英国	-
S. lanosoniveum	Cs0701	EU939525	中国台湾省	Salvinia molesta
S. lanosoniveum	CHE-CNRCB 373	KX686123	墨西哥	Diaphorina citri
S. lanosoniveum	CBS 123.42	MH856100	荷兰	Cibotium schiedei
S. lanosoniveum	vecl_02	KM035982	印度	Elaeagnus latifolia
S. lanosoniveum	vecl_01	KM035981	印度	Elaeagnus latifolia
S. lanosoniveum	CBS 531.72	MH860557	美国	Salvinia rotundifolia
S. lanosoniveum	CBS 321.72	MH860488	马来西亚	-
S. lanosoniveum	SSBG2	MG807436	俄罗斯	Coccus hesperidum
S. lanosoniveum	CBS 962.72	EF641862	-	-
S. lanosoniveum	YLAC-5	KY552635	中国	Inula aconitum
S. lanosoniveum	Tr3	MG026635	中国	Salvia miltiorrhiza
S. lanosoniveum	02502	KT878334	中国	支气管肺泡灌洗液
S. lanosoniveum	43A	MN860012	中国吉林省	Stemonitis splendens
S. lanosoniveum	43B	MN860013	中国吉林省	Stemonitis splendens
S. lanosoniveum	44A	MN860014	中国吉林省	Stemonitis splendens
S. lanosoniveum	44B	MN860015	中国吉林省	Stemonitis splendens
S. minatense	JCM 18178	AB603993	日本港区	Prunus yedoensis下的土壤
S. minatense	JCM 18176	AB603992	日本港区	Prunus yedoensis下的土壤
S. obclavatum	-	MH860859	-	-
S. subtropicum	JCM 18180	AB603990	日本小笠原岛	Cinnamomumpseudopedunculatum下的土壤
S. sympodiophorum	JCM 18184	AB604003	日本伊豆岛	Asplenium antiquum下的土壤

表1 本研究中用于系统发育分析的序列信息

Table 1 Sequences information used for phylogenetic analysis in this study

物种名	菌株名	登录号	国家/地区	分离来源
Cordyceps pseudomilitaris	NHJ6	AJ786589	-	-
Lecanicillium lecanii	CBS 101247	AJ292382	西印度群岛	Coccus viridis
Simplicillium aogashimaense	JCM 18167	AB604002	日本伊豆岛	Asplenium antiquum下的土壤
S. calcicola	LC5371	KU746705	中国贵州省	岩石
S. calcicola	LC5586	KU746706	中国贵州省	岩石
S. chinense	LC1345	JQ410324	中国	木材
S. cylindrosporum	JCM 18169	AB603989	日本小笠原岛	Cinnamomumpseudopedunculatum下的土壤
S. lamellicola	CBS 116.25	MH854806	英国	-
S. lamellicola	CBS:596.71	MH860281	英国	-
S. lanosoniveum	Cs0701	EU939525	中国台湾省	Salvinia molesta
S. lanosoniveum	CHE-CNRCB 373	KX686123	墨西哥	Diaphorina citri
S. lanosoniveum	CBS 123.42	MH856100	荷兰	Cibotium schiedei
S. lanosoniveum	vecl_02	KM035982	印度	Elaeagnus latifolia
S. lanosoniveum	vecl_01	KM035981	印度	Elaeagnus latifolia
S. lanosoniveum	CBS 531.72	MH860557	美国	Salvinia rotundifolia
S. lanosoniveum	CBS 321.72	MH860488	马来西亚	-
S. lanosoniveum	SSBG2	MG807436	俄罗斯	Coccus hesperidum
S. lanosoniveum	CBS 962.72	EF641862	-	-
S. lanosoniveum	YLAC-5	KY552635	中国	Inula aconitum
S. lanosoniveum	Tr3	MG026635	中国	Salvia miltiorrhiza
S. lanosoniveum	02502	KT878334	中国	支气管肺泡灌洗液
S. lanosoniveum	43A	MN860012	中国吉林省	Stemonitis splendens
S. lanosoniveum	43B	MN860013	中国吉林省	Stemonitis splendens
S. lanosoniveum	44A	MN860014	中国吉林省	Stemonitis splendens
S. lanosoniveum	44B	MN860015	中国吉林省	Stemonitis splendens
S. minatense	JCM 18178	AB603993	日本港区	Prunus yedoensis下的土壤
S. minatense	JCM 18176	AB603992	日本港区	Prunus yedoensis下的土壤
S. obclavatum	-	MH860859	-	-
S. subtropicum	JCM 18180	AB603990	日本小笠原岛	Cinnamomumpseudopedunculatum下的土壤
S. sympodiophorum	JCM 18184	AB604003	日本伊豆岛	Asplenium antiquum下的土壤

表2 发酵条件的因素和水平

Table 2 Factors and levels of fermentation condition

水平	因素
水平	A：麦芽糖	B：蛋白胨	C：KH₂PO₄
1	3%	0.1%	0.05%
2	4%	0.2%	0.10%
3	5%	0.3%	0.15%

图1 SL001在PDA培养基上培养14 d后的形态特征A：培养物的俯视图；B：培养物的背面；C：菌丝；D~G：瓶梗和瓶梗顶端的分生孢子；H：分生孢子。比例尺：C = 0.5 mm, D~F = 10 μm, G、 H = 5 μm；D、E、 G、 H用乳酸酚棉蓝溶液染色。

Fig. 1 Morphology of S. lanosoniveum on PDA medium after 14 days

图2 基于ITS序列构建的Simplicillium属系统发育树注：节点上标注了最大似然法自举值（>70%）和贝叶斯后验概率（≥95%）的支持度。该系统发育树以Lecanicillium lecanii 和Cordyceps pseudomilitaris为根序列。

Fig. 2 Phylogenetic relationships among the genus Simplicillium based on the ITS sequences data

图3 不同碳源对菌丝生物量和胞外多糖产量的影响A：不同碳源对菌丝生物量和胞外多糖产量的影响；B：不同碳源菌丝生长情况。**与***分别表示与同参数下的空白对照比差异在P<0.01和P<0.001水平上具有统计学意义。

Fig. 3 Effect of different carbon source on mycelial biomass and exopolysaccharides yield

图4 不同氮源对菌丝生物量和胞外多糖产量的影响A：不同氮源对菌丝生物量和胞外多糖产量的影响；B：不同氮源菌丝生长情况。**与***分别表示与同参数下的空白对照比差异在P<0.01和P<0.001水平上具有统计学意义。

Fig. 4 Effect of different nitrogen source on mycelial biomass and exopolysaccharides yield

图5 不同微量元素对菌丝生物量和胞外多糖产量的影响A：不同微量元素对菌丝生物量和胞外多糖产量的影响；B：不同微量元素菌丝生长情况。**与***分别表示与同参数下的空白对照比差异在P<0.01和P<0.001水平上具有统计学意义。

Fig. 5 Effect of different microelement on mycelial biomass and exopolysaccharides yield

表5 S. lanosoniveum.最佳发酵条件的正交实验设计

Table 5 Orthogonal experiment design for optimal submerged fermentation condition of S. lanosoniveum

序号	因素水平			菌丝生物量（g·100 mL^-1）
序号	A：麦芽糖	B：蛋白胨	C:KH₂PO₄	菌丝生物量（g·100 mL^-1）
1	1	1	1	0.70±0.18
2	1	2	2	0.70±0.09
3	1	3	3	0.81±0.26
4	2	1	2	0.97±0.21
5	2	2	3	1.11±0.10
6	2	3	1	1.33±0.11
7	3	1	3	0.88±0.05
8	3	2	1	0.85±0.22
9	3	3	2	1.07±0.14
k₁	0.737	0.850	0.960	—
k₂	1.137	0.887	0.970	—
k₃	0.933	1.070	0.877	—
r	0.400	0.220	0.093	—

表6 方差分析

Table 6 Analysis of variance

因素	SS	Df	F	P
麦芽糖（A）	0.240	2	80.000	*
蛋白胨（B）	0.083	2	27.667	*
KH₂PO₄（C）	0.003	2	1.000
误差	0.09	2
汇总	0.416	8

图6 pH对菌丝生物量和胞外多糖产量的影响注：***表示与pH 7.0处理组相比，差异在P<0.001水平有统计学意义。

Fig. 6 Effect of pH on mycelial biomass and exopolysaccharides yield

图7 加液量对菌丝生物量和胞外多糖产量的影响注：*表示与加液量100 mL处理组相比，差异在P<0.05水平有统计学意义。

Fig. 7 Effect of liquid volume on mycelial biomass and exopolysaccharides yield

图8 接种量对菌丝生物量和胞外多糖产量的影响注：***表示与接种量10%处理组相比，差异在P<0.001水平有统计学意义。

Fig. 8 Effect of inoculum volume on mycelial biomass and exopolysaccharides yield

参考文献 53

1	ZARE R, GAMS W.A revision of the Verticillium fungicola species complex and its affinity with the genus Lecanicillium [J].Mycol. Res., 2008, 112(7): 811-824.
2	SUNG G H, HYWEL-JONES N L, SUNG J M, et al.. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi[J]. Stud. Mycol., 2007, 57: 5-59.
3	LIU F, CAI L. Morphological and molecular characterization of a novel species of Simplicillium from China[J]. Cryptogam. Mycol. 2012, 33(2): 137-144.
4	WEI D P, WANASINGHE D N, HYDE K D, et al.. The genus Simplicillium [J]. MycoKeys, 2019, 60: 69-92.
5	CHEN W H, LIU C, HAN Y F, et al.. Three novel insect-associated species of Simplicillium (Cordycipitaceae,Hypocreales) from Southwest China[J]. MycoKeys, 2019, 58: 83-102.
6	GOMES A, PINHO D B, CARDEAL Z, et al.. Simplicillium coffeanum, a new endophytic species from Brazilian coffee plants, emitting antimicrobial volatiles[J]. Phytotaxa, 2018, 333(2): 188-198.
7	GREENGARTEN P J, TUININGA A R, MORATH S U,et al.. Occurrence of soil- and tick-borne fungi and related virulence tests for pathogenicity to Ixodes scapularis (Acari:Ixodidae)[J]. J. Med. Entomol., 2011, 48(2): 337-344.
8	HUBNER-CAMPOS R F, LELES R N, RODRIGUES J, et al.. Efficacy of entomopathogenic hypocrealean fungi against Periplaneta americana [J]. Parasitol. int., 2013, 62(6): 517-521.
9	NONAKA K, KAIFUCHI S, ŌMURA S, et al.. Five new Simplicillium species (Cordycipitaceae) from soils in Tokyo, Japan[J]. Mycoscience, 2013, 54(1): 42-53.
10	WARD N A, SCHNEIDER R W, AIME M C. Colonization of soybean rust sori by Simplicillium lanosoniveum [J]. Fungal Ecol., 2011, 4(5): 303-308.
11	CHEN R S, HUANG C C, LI J C, et al.. First report of Simplicillium lanosoniveum causing brown spot on Salvinia auriculata and S. molesta in Taiwan[J]. Plant Dis., 2008, 92(11): 1589.
12	ZHANG Z F, ZHOU S Y, EURWILAICHITR L, et al.. Culturable mycobiota from Karst caves in China ‍Ⅱ, with descriptions of 33 new species[J]. Fungal Divers., 2021, 106(1): 29-136.
13	王妮,谢映平,樊金华.桑白盾蚧病原真菌Simplicillium ianosoniveum的致病性[J].菌物学报,2016,35(5):559-568.
	WANG N, XIE Y P, FAN J H. Pathogenicity of Simplicillium ianosoniveum TYL001 isolated from Pseudaulacaspis pentagona [J]. Mycosystema, 2016, 35(5): 559-568.
14	LIM S Y, LEE S, KONG H G, et al.. Entomopathogenicity of Simplicillium lanosoniveum isolated in Korea[J]. Mycobiology, 2014, 42(4): 317-321.
15	ISHII M, TAKESHITA J, ISHIYAMA M, et al.. Evaluation of the pathogenicity and infectivity of entomopathogenic hypocrealean fungi,isolated from wild mosquitoes in Japan and Burkina Faso, against female adult Anopheles stephensi mosquitoes[J]. Fungal Ecol., 2015, 15: 39-50.
16	SKAPTSOV M, SMIRNOV S, KUTSEV M, et al.. Pathogenicity of Simplicillium lanosoniveum to Coccus hesperidum [J]. Ukr. J. Ecol., 2017, 7(4): 689-691.
17	GAUTHIER N W, MARUTHACHALAM K, SUBBARAO K V, et al.. Mycoparasitism of Phakopsora pachyrhizi, the soybean rust pathogen, by Simplicillium lanosoniveum [J]. Biol. Contr., 2014, 76: 87-94.
18	FUKUDA T, SUDOH Y, TSUCHIYA Y, et al.. Isolation and biosynthesis of preussin B, a pyrrolidine alkaloid from Simplicillium lanosoniveum [J]. J. Nat. Prod., 2014, 77(4): 813-817.
19	YU Y T, HE S H, ZHAO Q M. Isolation and identifcation of matrine-producing fungal endophytes from Sophora alopecuroides in Ningxia[J]. Sci. Agric. Sini., 2013, 46: 2643-2654.
20	ASGHER M, UROOJ Y, AHMAD QAMAR S, et al.. Improved exopolysaccharide production from Bacillus licheniformis MS3:optimization and structural/functional characterization[J]. Int. J. Biol. Macromol., 2020, 151: 984-992.
21	ASGHER M, AHMAD Q S, IQBAL H M N. Microbial exopolysaccharide-based nano-carriers with unique multi-functionalities for biomedical sectors[J]. Biologia, 2021, 76(2): 673-685.
22	BILAL M, GUL I, BASHARAT A, et al.. Polysaccharides-based bio-nanostructures and their potential food applications[J]. Int. J. Biol. Macromol., 2021, 176: 540-557.
23	MA A, AR A, NK A, et al.. Bioconversion of sugarcane molasses waste to high-value exopolysaccharides by engineered Bacillus licheniformis [J/OL]. Case Stud. Chem. Environ. Engin., 2021, 3: 100084[2024-10-15]. .
24	JIANG H, LUAN Z, FAN Z, et al.. Antibacterial,antibiofilm,and antioxidant activity of polysaccharides obtained from fresh sarcotesta of Ginkgo biloba: bioactive polysaccharide that can be exploited as a novel biocontrol agent[J]. Evid. Based Complem. Altern. Med., 2021, 2021: 5518403.
25	MOUSSA S H, TAYEL A A, AL-TURKI A I. Evaluation of fungal chitosan as a biocontrol and antibacterial agent using fluorescence-labeling[J]. Int. J. Biol. Macromol., 2013, 54: 204-208.
26	FU R J, CHENG R, WANG S M, et al.. Succinoglycan Riclin reshaped the soil microbiota by accumulating plant probiotic species to improve the soil suppressiveness on Fusarium wilt of cucumber seedlings[J]. Int. J. Biol. Macromol., 2021, 182: 1883-1892.
27	COSTA T M, LENZI J, SILVA F H, et al.. Identification and antigiardial activity of biocompounds produced in the Ganoderma lipsiense Mycelium in submerged fermentation[J]. Nat. Prod. Res., 2021, 35(23): 5530-5534.
28	WANG X L, ZHANG L L, CHEN N, et al.. The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation[J]. Int. J. Biol. Macromol., 2021, 191: 377-384.
29	AHMAD QAMAR S, ASGHER M, BILAL M. Sustainable production,optimization,and partial characterization of exopolysaccharides by Macrococcus brunensis [J]. Waste Biomass Valor., 2021, 12(12): 6847-6859.
30	FENG J, FENG N, TANG Q, et al.. Development and optimization of the triterpenoid and sterol production process with Lingzhi or reishi medicinal mushroom, Ganoderma lucidum strain G0017 (Agaricomycetes), in liquid submerged fermentation at large scale[J]. Int. J. Med. Mushrooms, 2021, 23(3): 43-53.
31	ROGERS S O, BENDICH A J. Extraction of total cellular DNA from plants, algae and fungi[J]. Plant Mol. Biol. Man., 1994(1): 183-190.
32	WHITE T J, BRUNS T, LEE S, et al.. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[J]. PCR Protoc. Guide Methods Appl., 1990, 18(1): 315-322.
33	LIU D, GOFFINET B, ERTZ D, et al.. Circumscription and phylogeny of the Lepidostromatales (lichenized Basidiomycota) following discovery of new species from China and Africa[J]. Mycologia, 2017, 109(5): 730-748.
34	KUMAR S, STECHER G, TAMURA K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Mitochondrial DNA Resour., 2016, 33(7): 1870-1874.
35	KATOH K, STANDLEY D M. A simple method to control over-alignment in the MAFFT multiple sequence alignment program[J]. Bioinformatics, 2016, 32(13): 1933-1942.
36	STAMATAKIS A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies[J]. Bioinformatics, 2014, 30(9): 1312-1313.
37	HUELSENBECK J P, RONQUIST F. MRBAYES: Bayesian inference of phylogenetic trees[J]. Bioinformation, 2001, 17(8): 754-755.
38	POSADA D. jModelTest: phylogenetic model averaging[J]. Mol. Biol. Evol., 2008, 25(7): 1253-1256.
39	DUBOIS M, GILLES K A, HAMILTON J K, et al.. Colorimetric method for determination of sugars and related substances[J]. Anal. Chem., 1956, 28(3): 350-356.
40	KIM H O, YUN J W. A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures[J]. J. Appl. Microbiol., 2005, 99(4): 728-738.
41	ENSHASY H A, ELSAYED E A, SUHAIMI N, et al.. Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system[J/OL]. BMC Biotechnol., 2018, 18(1): 71[2024-10-15]..
42	SULEIMENOVA Z, AKHMETSADYKOV N, KALIEVA A, et al.. Effect of different cultural conditions for phytase production by Aspergillus niger in submerged fermentation [J]. Adv. Enzyme Res., 2016, 4(2): 62-67.
43	SINGH V, HAQUE S, NIWAS R, et al.. Strategies for fermentation medium optimization: an in-depth review[J/OL]. Front. Microbiol., 2016, 7: 2087[2024-10-15]. .
44	AMERI SHAH REZA M, VAHIDI H, KOBARFARD F. Optimization of growth conditions of Lentinus edodes Mycelium and polysaccharides on walnut shell by-products using response surface analysis[J]. Iran. J. Pharm. Res., 2018, 17(4): 1509-1522.
45	XIA Y, WANG Y, ZHANG B, et al.. Effect of cultural conditions on antrodin C production by basidiomycete Antrodia camphorata in solid-state fermentation[J]. Biotechnol. Appl. Biochem., 2014, 61(6): 724-732.
46	BAISWAR P, NGACHAN S V, RYMBAI H, et al.. Simplicillium lanosoniveum, a hyperparasite on Aecidium elaeagni-latifoliae in India[J/OL]. Australas. Plant Dis. Notes, 2014, 9(1): 144[2024-10-15]. .
47	DONG Q, DONG R, XING X, et al.. A new antibiotic produced by the Cyanobacterium-symbiotic fungus Simplicillium lanosoniveum [J]. Nat. Prod. Res., 2018, 32(11): 1348-1352.
48	WARD N A, ROBERTSON C L, CHANDA A K, et al.. Effects of Simplicillium lanosoniveum on Phakopsora pachyrhizi, the soybean rust pathogen, and its use as a biological control agent[J]. Phytopathology, 2012, 102(8): 749-760.
49	DE VUYST L, VANDERVEKEN F, VAN DE VEN S, et al.. Production by and isolation of exopolysaccharides from Streptococcus thermophilus grown in a milk medium and evidence for their growth-associated biosynthesis[J]. J. Appl. Microbiol., 1998, 84(6): 1059-1068.
50	LIU G Q, WANG X L. Optimization of critical medium components using response surface methodology for biomass and extracellular polysaccharide production by Agaricus blazei [J]. Appl. Microbiol. Biotechnol., 2007, 74(1): 78-83.
51	杨莲芳.川牛膝枝枯病病原鉴定及生物学特性研究[D].雅安:四川农业大学,2009.
52	张树强. 应用单孢分离技术选育鸡腿菇优良品种[D]. 塔里木大学, 2012.
53	SCHÉMAEZA B, SOMDA I, SEREME P, et al.. Effects of temperature and pH on Mycelium growth of Phoma sorghina (Sacc.) Boerema Dorenbosch and Van Kesteren in vitro [J]. Pak. J. Biol. Sci., 2013, 16(24): 2054-2057.

基于菌丝生物量和胞外多糖产量的Simplicillium lanosoniveum液体发酵培养条件优化

Optimization of Liquid Fermentation Conditions for Mycelial Biomass and Exopolysaccharide Production of Simplicillium lanosoniveum

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