关键词: 陆地棉, 纤维优势表达, GhRACK1, 克隆与分析

Abstract: Fiber quality improvement is one of the main targets in cotton breeding. The discovery of specific or dominant expressed gene  in fiber is the key factor that improved fiber quality by using genetic engineering strategy. In this study, we used G. hirsutum var. Sumian12 as a starting material to clone the full-length cDNA of GhRACK1 gene by RACE techniques according known EST sequence. The deduced amino acid sequence of GhRACK1 was highly homologous to the other known RACK1 proteins and contained 4 cascade WD motifs, which belonged to WD40 family. GhRACK1 protein and known RACE1 had similar three-dimensional structure by PDB simulation. qPCR results indicated that the expression level of GhRACK1 in fiber was more than 20-fold compared with leaf. This study provided new gene resource for cotton fiber improvement.

Key words: Gossypium hirsutum, fiber-superiority expression, GhRACK1, cloning and sequence analysis