木质素生物合成翻译后修饰调控研究进展

doi:10.19586/j.2095-2341.2024.0035

生物技术进展 ›› 2024, Vol. 14 ›› Issue (4): 519-528.DOI: 10.19586/j.2095-2341.2024.0035

• 进展评述 • 上一篇

木质素生物合成翻译后修饰调控研究进展

张昊¹^,²(), 陈亚娟²(), 姜廷波¹, 周博如¹(), 王宏芝²()

^1.东北林业大学林学院，哈尔滨 150040
^2.北京市农林科学院生物技术研究所，北京 100089

收稿日期:2024-02-28 接受日期:2024-03-27 出版日期:2024-07-25 发布日期:2024-08-07
通讯作者: 周博如,王宏芝
作者简介:张昊 E-mail：2891337761@qq.com
陈亚娟 E-mail：chenyajuan@baafs.net.cn第一联系人：张昊和陈亚娟为共同第一作者。
基金资助:
北京市农林科学院青年科学基金项目(QNJJ202123);北京市农林科学院科技创新能力建设专项(KJCX20230203)

Advances on the Post-translational Modifications Regulating of Lignin Biosynthesis

Hao ZHANG¹^,²(), Yajuan CHEN²(), Tingbo JIANG¹, Boru ZHOU¹(), Hongzhi WANG²()

^1.School of Forestry，Northeast Forestry University，Harbin 150040，China
^2.Institute of Biotechnology，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100089，China

Received:2024-02-28 Accepted:2024-03-27 Online:2024-07-25 Published:2024-08-07
Contact: Boru ZHOU,Hongzhi WANG

摘要/Abstract

摘要：

蛋白质翻译后修饰（post-translational modifications，PTMs）在植物生长发育过程中具有十分重要的作用，它们通过调节蛋白质的结构、稳定性以及活性，从而影响植物的生长发育以及响应环境胁迫的能力。木质素生物合成途径及其上游转录调控机制目前已研究得较为清楚，但翻译后修饰研究较少。综述了木质素生物合成翻译后修饰调控的最新研究进展，重点介绍了磷酸化、泛素化、糖基化和S-亚硝基化4类重要的翻译后修饰对木质素合成关键酶和转录因子的调控机制，旨在深化对木质素生物合成调控机理的认知，并为深入理解植物木质素合成的精准时空调控机制提供参考和启发。

关键词: 木质素, 翻译后修饰, 磷酸化, 泛素化

Abstract:

Protein post-translational modifications （PTMs） play a crucial role in plant growth and development. They significantly impact plant growth， development， and the ability to adapt to environmental stresses by fine-tuning the structure， stability， and activity of proteins. While the lignin biosynthetic pathway and its upstream transcriptional regulation have been extensively investigated， research on PTMs in this context remains relatively limited. This review summarized the latest advancements in understanding the regulation of lignin biosynthesis by PTMs， particularly focusing on four significant types： phosphorylation， ubiquitination， glycosylation， and S-nitrosylation. It highlighted the regulatory mechanisms of these PTMs on key enzymes and transcription factors involved in lignin biosynthesis， aiming to enhance our comprehension of the regulatory networks governing lignin biosynthesis and provide valuable insights and references for the precise spatiotemporal modulation of this crucial process in plants.

Key words: lignin, post-translational modifications, phosphorylation, ubiquitination

中图分类号:

Q945.4

张昊, 陈亚娟, 姜廷波, 周博如, 王宏芝. 木质素生物合成翻译后修饰调控研究进展[J]. 生物技术进展, 2024, 14(4): 519-528.

Hao ZHANG, Yajuan CHEN, Tingbo JIANG, Boru ZHOU, Hongzhi WANG. Advances on the Post-translational Modifications Regulating of Lignin Biosynthesis[J]. Current Biotechnology, 2024, 14(4): 519-528.

图/表 4

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蛋白名称	物种	翻译后修饰种类	翻译后修饰作用	参考文献
UBC34	毛白杨（Populus tomentosa）	泛素化	UBC34的泛素化降低转录因子MYB221的活性，调节木质素合成	[18]
KFB1、KFB20、KFB39、KFB50	拟南芥（Arabidopsis thaliana）	泛素化	KFB的泛素化影响PAL1蛋白质稳定性，调控苯丙烷合成	[19]
SAG1	拟南芥（Arabidopsis thaliana）	泛素化	SAG1的泛素化影响PAL1蛋白质稳定性，调控木质素合成	[20]
PeKFB9	Phyllostachys edulis	泛素化	PeKFB9的泛素化介导PePRX72-1降解，调控木质素单体聚合	[21]
ULCS1	拟南芥（Arabidopsis thaliana）	泛素化	调控木质素合成，RNAi植株花药壁U-型次生细胞壁沉积缺失	[22]
AldOMT2	毛果杨（Populus trichocarpa）	磷酸化	Ser¹²³或 Ser¹²磷酸化导致AldOMT2蛋白丧失催化活性	[23]
PAL2	毛果杨（Populus trichocarpa）×美洲黑杨（Populus deltoides）	磷酸化	磷酸化降低了PAL2蛋白质稳定性	[24]
PtMYB4	火炬松（Pinus taeda L.）	磷酸化	PtMYB4被丝裂原激活蛋白激酶PtMAPK6 磷酸化，磷酸化的PtMYB4 转录激活活性明显提高	[25]
LTF1	拟南芥（Arabidopsis thaliana）	磷酸化	磷酸化降低了LTF1蛋白稳定性，解除或减弱了其对木质素单体合成基因的转录抑制作用，进而激活木质素合成	[26]
OsRAI1	水稻（Oryza sativa）	磷酸化	磷酸化激活OsRAI1的转录激活活性，进而诱导PAL1等基因的转录，诱导植物防御反应	[27]
PXP1、PXP2、PXP3、PXP4、PXP5、PXP6	毛果杨（Populus trichocarpa）	糖基化	不明确	[28]
PRX	百日草（Zinnia elegans jacq.）	糖基化	糖基化改变PRX的催化效率	[29]
VND7	拟南芥（Arabidopsis thaliana）	S-亚硝基化	S-亚硝基化降低了VND7对次生细胞壁合成基因的反式激活活性	[30]

蛋白名称	物种	翻译后修饰种类	翻译后修饰作用	参考文献
UBC34	毛白杨（Populus tomentosa）	泛素化	UBC34的泛素化降低转录因子MYB221的活性，调节木质素合成	[18]
KFB1、KFB20、KFB39、KFB50	拟南芥（Arabidopsis thaliana）	泛素化	KFB的泛素化影响PAL1蛋白质稳定性，调控苯丙烷合成	[19]
SAG1	拟南芥（Arabidopsis thaliana）	泛素化	SAG1的泛素化影响PAL1蛋白质稳定性，调控木质素合成	[20]
PeKFB9	Phyllostachys edulis	泛素化	PeKFB9的泛素化介导PePRX72-1降解，调控木质素单体聚合	[21]
ULCS1	拟南芥（Arabidopsis thaliana）	泛素化	调控木质素合成，RNAi植株花药壁U-型次生细胞壁沉积缺失	[22]
AldOMT2	毛果杨（Populus trichocarpa）	磷酸化	Ser¹²³或 Ser¹²磷酸化导致AldOMT2蛋白丧失催化活性	[23]
PAL2	毛果杨（Populus trichocarpa）×美洲黑杨（Populus deltoides）	磷酸化	磷酸化降低了PAL2蛋白质稳定性	[24]
PtMYB4	火炬松（Pinus taeda L.）	磷酸化	PtMYB4被丝裂原激活蛋白激酶PtMAPK6 磷酸化，磷酸化的PtMYB4 转录激活活性明显提高	[25]
LTF1	拟南芥（Arabidopsis thaliana）	磷酸化	磷酸化降低了LTF1蛋白稳定性，解除或减弱了其对木质素单体合成基因的转录抑制作用，进而激活木质素合成	[26]
OsRAI1	水稻（Oryza sativa）	磷酸化	磷酸化激活OsRAI1的转录激活活性，进而诱导PAL1等基因的转录，诱导植物防御反应	[27]
PXP1、PXP2、PXP3、PXP4、PXP5、PXP6	毛果杨（Populus trichocarpa）	糖基化	不明确	[28]
PRX	百日草（Zinnia elegans jacq.）	糖基化	糖基化改变PRX的催化效率	[29]
VND7	拟南芥（Arabidopsis thaliana）	S-亚硝基化	S-亚硝基化降低了VND7对次生细胞壁合成基因的反式激活活性	[30]

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木质素生物合成翻译后修饰调控研究进展

Advances on the Post-translational Modifications Regulating of Lignin Biosynthesis

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