氨氮降解菌株的筛选及降解性能研究

doi:10.19586/j.2095-2341.2022.0024

生物技术进展 ›› 2022, Vol. 12 ›› Issue (5): 746-753.DOI: 10.19586/j.2095-2341.2022.0024

氨氮降解菌株的筛选及降解性能研究

张国伟¹(), 靳静晨², 李晨晨¹, 高宇轩¹, 高雅娟¹, 张闻天¹, 靳永胜¹()

^1.北京农学院生物与资源环境学院，北京 102206
^2.北京环氧环保科技发展有限公司，北京 100101

收稿日期:2022-03-04 接受日期:2022-05-26 出版日期:2022-09-25 发布日期:2022-09-30
通讯作者: 靳永胜
作者简介:张国伟 E-mail: 1728047527@qq.com;
基金资助:
校企合作生物除臭技术研发项目(2014115024);北京农学院学位与研究生教育改革与发展项目(50765160201018)

Isolation and Identification of Ammonia Nitrogen Degradation Fungus and Study on Deamination Characteristics

Guowei ZHANG¹(), Jingchen JIN², Chenchen LI¹, Yuxuan GAO¹, Yajuan GAO¹, Wentian ZHANG¹, Yongsheng JIN¹()

^1.College of Bioscience and Resources Environment，Beijing University of Agriculture，Beijing 102206，China
^2.Beijing Epoxy Environmental Protection Technology Development Co. ，Ltd，Beijing 100101，China

Received:2022-03-04 Accepted:2022-05-26 Online:2022-09-25 Published:2022-09-30
Contact: Yongsheng JIN

摘要/Abstract

摘要：

为解决氨氮积累导致水体污染的问题，从北京南宫垃圾堆肥厂的垃圾渗滤液中筛选出1株具有氨氮降解能力的菌株Z-5。通过对该菌株的形态观察以及ITS基因序列同源性对比分析，鉴定该菌株为褐红篮状菌（Talaromyces pinophilus），命名为褐红篮状菌Z-5。进一步分析筛选菌株Z-5在不同培养条件（接种量、氨氮初始浓度、碳源、pH）下各单因素以及多因素对氨氮降解性能的影响，并进行了二元分布和三因素三水平的正交试验。结果表明，当接种量为1%、氨氮初始浓度为100 mg·L^-1、碳源为红糖以及pH为7.0时，氨氮去除率达到了94.75%，效果最佳。研究结果为垃圾废水处理中氨氮的去除奠定了基础。

关键词: 褐红篮状菌, 氨氮降解菌株, 降解性能, 二元分布, 正交试验

Abstract:

In order to solve the problem of water pollution caused by ammonia nitrogen accumulation， the strain Z-5 with ammonia nitrogen degradation ability was screened from the landfill leachate of Beijing Nangong Garbage Compost Plant. The strain was identified as Talaromycespinophilus through the morphological observation of the strain and the comparative analysis of the homology of the ITS gene sequence， which was further researched and named as Talaromyces pinophilus Z-5. The effects of single and multiple factors on ammonia nitrogen degradation performance of Z-5 were studied under different culture conditions （inoculation amount， initial concentration of ammonia nitrogen， carbon source， pH）. The orthogonal tests of binary distribution and three factors and three levels were carried out. The results showed that best ammonia nitrogen degradation rate of ammonia nitrogen was 94.75%， which was the highest， and the condition of the inoculum size was 1%， the initial concentration of ammonia nitrogen was 100 mg·L^-1， the carbon source was brown sugar and the pH was 7.0. This study laid a foundation for the removal of ammonia nitrogen in practical wastewater treatment.

Key words: Talaromyces pinophilus, ammonia nitrogen degradation fungus, deamination characteristics, bivariate distribution, orthogonal experiment

中图分类号:

S917.1

张国伟, 靳静晨, 李晨晨, 高宇轩, 高雅娟, 张闻天, 靳永胜. 氨氮降解菌株的筛选及降解性能研究[J]. 生物技术进展, 2022, 12(5): 746-753.

Guowei ZHANG, Jingchen JIN, Chenchen LI, Yuxuan GAO, Yajuan GAO, Wentian ZHANG, Yongsheng JIN. Isolation and Identification of Ammonia Nitrogen Degradation Fungus and Study on Deamination Characteristics[J]. Current Biotechnology, 2022, 12(5): 746-753.

图/表 10

表1 因素水平表

Table 1 The factors and levels scale

水平	因素
水平	A：氨氮初始浓度/（mg·L^-1）	B：碳源	C：pH
1	100	葡萄糖	3.0
2	250	蔗糖	5.0
3	500	红糖	7.0

图1 菌株Z-5在光学显微镜下的形态注：放大倍数为1 000倍。

Fig. 1 Morphology of strain Z-5 under optical microscope

图2 菌株Z-5的ITS基因序列系统发育树

Fig. 2 Phylogenetic tree of ITS gene sequence of Z-5 species

图3 接种量对菌株Z-5氨氮降解率的影响注：同一时间的不同小写字母表示不同处理组差异在P<0.05水平有统计学意义。

Fig. 3 Effects of inoculation amount on ammonia degradation rate of Z-5

图4 氨氮初始浓度对菌株Z-5氨氮降解率的影响注：同一时间的不同小写字母表示不同处理组差异在P<0.05水平有统计学意义。

Fig. 4 Effect of initial concentration of ammonia nitrogen on ammonia degradation rate of Z-5

图5 氨氮初始浓度对菌株Z-5氨氮绝对降解量的影响注：同一时间的不同小写字母表示不同处理组差异在P<0.05水平有统计学意义。

Fig. 5 Effect of initial ammonia nitrogen concentration on absolute degradation of ammonia nitrogen by strain Z-5

图6 碳源对Z-5菌株氨氮浓度的影响注：不同小写字母表示不同处理组差异在P<0.05水平有统计学意义。

Fig. 6 Effect of carbon source on ammonia concentration of Z-5

图7 pH对Z-5菌株氨氮降解率的影响注：同一时间的不同小写字母表示不同处理组差异在P<0.05水平有统计学意义。

Fig. 7 Effect of pH on ammonia degradation rate of Z-5

图8 pH和氨氮初始浓度之间的二元分布

Fig. 8 Bivariate distribution of pH and initial concentration of ammonia nitrogen

表2 正交试验结果

Table 2 The results of orthogonal experiments

因素	A：氨氮初始浓度	B：碳源	C：pH	氨氮降解率/%
1	A1	B1	C1	90.21
2	A1	B2	C2	92.34
3	A1	B3	C3	94.75
4	A2	B1	C2	87.84
5	A2	B2	C3	90.52
6	A2	B3	C1	89.58
7	A3	B1	C3	62.34
8	A3	B2	C1	61.94
9	A3	B3	C2	63.33
K₁	277.30	240.39	241.73
K₂	267.94	244.80	243.51
K₃	187.61	247.66	247.61
R	89.69	7.27	5.88

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氨氮降解菌株的筛选及降解性能研究

Isolation and Identification of Ammonia Nitrogen Degradation Fungus and Study on Deamination Characteristics

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