关键词: 鸡&kappa, 干扰素, 家蚕杆状病毒表达系统, 抗病毒活性检测

Abstract: Interferons as an integral component of the hosts innate immune system are considered as the first line of defense against invading viral pathogens.  Keeping in view, the present study was expected to express and profile the chicken interferon-κ (chIFN-κ) using silkworm baculovirus expression vector system (BEVS). Firstly, based on the reported sequences, the gene sequence of chIFN-κ was codon-optimized, and the recombinant baculovirus for expression was successfully constructed using co-transfection technology. Then, the recombinant baculovirus harboring chIFN-κ was infected in silkworm larva, and a huge quantity of chIFN-κ expression products were successfully obtained. chIFN-κ was detected and verified by Western blot assay using specific antibodies. Cytopathic inhibition assay was performed to validate the effect of chIFN-κ on VSV-GFP-infected primary chicken fibroblasts（CEF）. The results revealed that the antiviral effect of chIFN-κ could reach (1.04±0.23)×107 IU·mL-1 or more. The successful expression strategy of biologically active and high-potency chIFN-κ provided significant experimental data. These experimental findings may lead to the development of future vaccines for the poultry industry.

Key words: chicken &kappa, interferon, silkworm baculovirus expression system, antiviral activity assay