关键词: 带鱼, 肠道内容物菌群, 16S rRNA测序, 支原体

Abstract: Hairtail fish (Trichiurus spp.) is an important economic fish in the East China Sea. In order to identify its own pathogenic microorganisms, we analyzed the intestinal contents flora of hairtail fish. Intestinal contents flora DNA extraction of hairtail fish captured in the East China Sea were collected, Ion S5TM XL high-throughput sequencing platform and bioinformatics analysis were used to construct the 16S rRNA v3-v4 gene sequencing library of 10 samples. 1 221 OTUs (operational taxonomic units) were obtained from 10 samples, which were classified into 28 phylum and 345 genera. At the phylum level, the abundance of Tenericutes and Proteobacteria is relatively high, which are 51.30% and 29.58% respectively. At the genus level, Mycoplasma and Vibrio account for a relatively high percentage, with relative abundance of 50.95% and 13.79%, respectively. The results showed that Mycoplasma in the intestinal contents flora of hairtail fish is relatively high, and there is a possibility of cross-contamination in the food processing. Therefore, it is necessary to be vigilant in the relevant production process, and certain measures should be taken to control Mycoplasma contamination. The results was excepted to provide reference for the study on the discovery, prevention and control of the new pathogen.Trichiurus spp.; intestinal contents flora; 16S rRNA sequencing; Mycoplasma

Key words: Trichiurus spp., intestinal contents flora, 16S rRNA sequencing, Mycoplasma