小麦-鹅观草第一部分同源群染色体渗入系鉴定与基因组归属分析

doi:10.19586/j.2095-2341.2021.0115

生物技术进展 ›› 2021, Vol. 11 ›› Issue (5): 567-573.DOI: 10.19586/j.2095-2341.2021.0115

• 赤霉病抗源及种质创新 • 上一篇下一篇

小麦-鹅观草第一部分同源群染色体渗入系鉴定与基因组归属分析

王仪威(), 冯祎高(), 刘润然, 卢春甜, 曹爱忠, 张瑞奇()

南京农业大学农学院，江苏省现代作物生产协同创新中心，南京 210095

收稿日期:2021-06-15 接受日期:2021-06-28 出版日期:2021-09-25 发布日期:2021-10-08
通讯作者: 张瑞奇
作者简介:王仪威与冯祎高为本文共同第一作者。王仪威 E-mail：2020801240@njau.edu.cn
冯祎高 E-mail：ygfeng@njau.edu.cn
基金资助:
江苏省农业自主创新项目〔CX（19）1001〕;江苏省自然科学基金项目(BK20181316)

Introgression and Characterization of the Homologous Group 1 Chromosomes from Roegneria kamoji into Common Wheat

Yiwei WANG(), Yigao FENG(), Runran LIU, Chuntian LU, Aizhong CAO, Ruiqi ZHANG()

Jiangsu Collaborative Innovation Center for Modern Crop Production，College of Agronomy，Nanjing Agricultural University，Nanjing 210095，China

Received:2021-06-15 Accepted:2021-06-28 Online:2021-09-25 Published:2021-10-08
Contact: Ruiqi ZHANG

摘要/Abstract

摘要：

鹅观草（Roegneria kamoji，2n=42，SSHHYY）是小麦异源六倍体野生近缘种，对小麦赤霉病具有良好抗性，是改良小麦赤霉病抗性的重要遗传资源。通过远缘杂交，将鹅观草第一部分同源群染色体上的抗赤霉病基因Fhb6导入普通小麦。由于第一部分同源群染色体包含1S、1H和1Y三条染色体，为研究这些同源染色体对小麦赤霉病抗性的影响，筛选出4个鹅观草第一部分同源群染色体特异分子标记，通过PCR扩增鹅观草属不同野生种的基因组DNA，明确了抗赤霉病Fhb6基因位于鹅观草1Y#1染色体。进一步利用分子细胞遗传学技术从中国春与鹅观草的后代中选育出5份涉及鹅观草1Y#2和1S#2染色体的渗入系材料。其中：21RK?1为二体异代换系DS1Y#2(1A)，21RK?2为二体异代换系DS1S#2（1D），21RK?3为二体异附加系DA1S#2，21RK?4为1S#2和TW·1S#2S的双单体附加系，21RK?5为纯合TW·1S#2S易位系。这些新种质为小麦抗赤霉病基因的发掘及遗传改良奠定了基础。

关键词: 小麦赤霉病, 鹅观草, Fhb6, 1Y染色体, 1S染色体

Abstract:

Roegneria kamoji （2n=42， SSHHYY） is a hexaploid wild relatives of common wheat. Due to high resistance level of Fusarium head blight （FHB）， R. kamoji is regarded as a potential germplasm to explore FHB?resistant genes. The FHB resistance gene Fhb6 located on the homoeologous group 1 chromosome of R. kamoji had been introduced into common wheat through hybridization. The homoeologous group 1 chromosomes of R. kamoji included 1S， 1H or 1Y chromosome. In order to understand the effect of homoeologous chromosome on resistance of FHB， four STS markers specific to homoeologous group 1 chromosomes of R. kamoji werescreened. Subsequently， these markers were used to amplify the DNA samples of the disomic substitution line DS1RK#1（1A） carrying Fhb6 and the other relatives with different genomes. Results indicated that Fhb6 was located on 1Y#1 chromosome. Five new wheat?R. kamoji introgression lines involving 1Y#2 and 1S#2 chromosomes were identified from the backcross progeny of the second hybrid between Chinese Spring and R. kamoji. Of which， 21RK?1 was the disomic substitution line DS1Y#2（1A）， 21RK?2 was the disomic substitution line DS1S#2（1D）， 21RK?3 was the disomic addition line DA1S#2， 21RK?4 was the double monosomic addition line of 1S#2 and TW·1S#2S， and 21RK?5 was the homologous TW·1S#2S translocation line. These new germplasm resources and the specific molecular markers developed in the present study provided the foundation for the genetic improvement of wheat resistance to Fusarium head blight.

Key words: Fusarium head blight （FHB）, Roegneria kamoji, Fhb6 gene, chromosome 1Y, chromosome 1S

中图分类号:

S512

王仪威, 冯祎高, 刘润然, 卢春甜, 曹爱忠, 张瑞奇. 小麦-鹅观草第一部分同源群染色体渗入系鉴定与基因组归属分析[J]. 生物技术进展, 2021, 11(5): 567-573.

Yiwei WANG, Yigao FENG, Runran LIU, Chuntian LU, Aizhong CAO, Ruiqi ZHANG. Introgression and Characterization of the Homologous Group 1 Chromosomes from Roegneria kamoji into Common Wheat[J]. Current Biotechnology, 2021, 11(5): 567-573.

图/表 5

表1 鹅观草第一部分同源群染色体特异分子标记

Table 1 Specific molecular markers of homologous group chromosomes1 in R. kamoji

标记	引物序列(5′→3′)	小麦染色体定位	鹅观草染色体定位	退火温度/℃
CINAU27	F: 5′‑TTGTTGCTGCCTGTGAAAAC‑3′ R: 5′‑ACCAGTTAGCGTTGCCAGAT‑3′	1AS、1BS、1DS	1SS、1YS	58
CINAU190	F: 5′‑ACCAGAACTCCAAGCTCTCC‑3′ R: 5′‑CCCTCCGCGTAGATCTTGTA‑3′	1AS、1BS、1DS	1SS、1HS、1YS	60
CINAU207	F: 5′‑GTTTCAGGGAGGCTGCATTG‑3′ R: 5′‑CCCGAGCACTCATTAACAACC‑3′	1AS、1BS、1DS	1SS、1HS、1YS	60
CINAU493	F：5′‑GGCCTTTCATTTGCTTACCA‑3′ R：5′‑CATGGAGGACCACGAAGTCT‑3′	1AL、1BL、1DL	1SL、1HL、1YL	55

图1 分子标记CINAU27在普通小麦及其近缘属种PCR扩增产物

Fig.1 PCR productions amplified by marker CINAU27 in wheat and its relatives

图2 基因组特异分子标记在5份小麦-鹅观草渗入系材料的PCR扩增结果A:引物CINAU27 PCR 扩增产物电泳结果；B: 引物CINAU190 PCR 扩增产物电泳结果；C: 引物CINAU207 PCR 扩增产物电泳结果；D: 引物CINAU493 PCR 扩增产物电泳结果

Fig. 2 PCR productions of five wheat?R. kamoji introgression lines amplified by using four STS markers

图3 小麦-鹅观草渗入系材料的GISH/FISH分析A：小麦-鹅观草二体异代换系DS1Y#1(1A)；B：21RK?1；C：21RK?2；D：21RK?3；E：21RK?4；F：21RK?5；G：FISH

Fig. 3 GISH/FISH analysis of six wheat?R. kamoji introgression lines

图4 中国春及中国春-鹅观草渗入系穗型A：中国春穗型；B：中国春-鹅观草二体异代换系21RK?2；C：中国春-鹅观草二体异附加系21RK?3

Fig.4 Spikes of Chinese Spring and CS? R. kamoji introgression lines

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小麦-鹅观草第一部分同源群染色体渗入系鉴定与基因组归属分析

Introgression and Characterization of the Homologous Group 1 Chromosomes from Roegneria kamoji into Common Wheat

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