生物技术进展 ›› 2011, Vol. 1 ›› Issue (6): 432-435.DOI: 10.3969/j.issn.2095-2341.2011.06.09

• 研究论文 • 上一篇    下一篇

橙色粘球菌子实体形成过程的显微观察

曾振顺1,2,谭志远2,张鲜娇1,朱红惠1   

  1. 1.广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广州 510070;
    2.华南农业大学农学院, 广州 510642
  • 收稿日期:2011-10-25 出版日期:2011-12-25 发布日期:2011-11-25
  • 通讯作者: 朱红惠,研究员,博士,主要从事特殊生境的微生物分离与鉴定研究。E-mail:zhuhonghui66@yahoo.com.cn
  • 作者简介:曾振顺,硕士研究生,研究方向为粘细菌的分离鉴定。
  • 基金资助:

    国家自然科学基金项目(31170009),广东省自然科学基金项目(10151007002000008),新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题重点项目(BRZD1004)资助。

Observation of the Fruiting Body Formation of Myxococcus Fulvus by Microscope Technic

ZENG Zhen-shun, TAN Zhi-yuan, ZHANG Xian-jiao, ZHU Hong-hui   

  1. 1.Guangdong Institute of Microbiology,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, China;
    2.College of Agriculture,South China Agricultural University, Guangzhou 510642, China
  • Received:2011-10-25 Online:2011-12-25 Published:2011-11-25

摘要: 利用体式显微镜(stereomicroscope,SMC)和扫描电镜(scanning electron microscope,SEM)对橙色粘球菌(Myxococcus fulvus)的子实体形成过程进行为期8 d的生长周期观察,揭示橙色粘球菌由营养体逐渐聚集变成子实体的过程;SMC观察得到粘细菌菌落形成聚集以及颜色逐渐变深,并最后形成突起的鲜亮的橙色球状子实体。SEM观察得到明显的群体细胞定向运动以及细胞堆积,粘细菌细胞由杆状营养体向球状粘孢子变化,并最终形成由粘孢子组成的球状子实体。

关键词: 橙色粘球菌, 子实体, 生活史

Abstract: Life cycle of Myxococcus fulvus was observed with stereomicroscope (SMC) and scanning electron microscope (SEM) by culture it within eight days. The results revealed the process of vegetative cells moveing thgether to form the fruiting body. SMC observation indicated the swarm of Myxococcus fulvus gathered around to form the fruiting body and the color of them turned into bright orange. SEM observation indicated that a pile of cells moved together toward one diretion and heaped up to build up the huge fruiting body. Also, the vegetation cells turnned into the spherical myxospores.

Key words: Myxococcus fulvus, fruiting body, life history