关键词: 蔗糖合成酶, Zmsh1, 转基因烟草, 生物量, 基因应用价值评估

Abstract: Sucrose synthetase (SuSy) is a crucial enzyme family regulating sucrose metabolism in plants. SH1 is one of the subtypes of SuSy in maize, and its encoding gene (Zmsh1) plays a major role in the catalytic activity of SuSy. Thus, the evaluation of Zmsh1 in biotech breeding has attracted a great deal of  attention in recent years. In this study, we transformed Zmsh1 into tobacco by Agrobacterium-mediated method. The results showed that, enzyme activity of SH1 in the transgenic events exhibited an average of 35% higher than the wild type, and contents of the key metabolites such as sucrose and fructose increased about 23% and 28% respectively in the root and stem of the transgenic events. At the meantime, the transformation of Zmsh1 significantly enhanced the biomass of transgenic tobacco. This preliminary study provided important reference for proving concept of Zmsh1 in maize biotech breeding.

Key words: SuSy, Zmsh1, transgenic tobacco, biomass, prove concept of gene