关键词: 酿酒酵母, CIT2基因, 基因敲除, 木糖代谢

Abstract: Xylose fermentation is one of the bottleneck of cellulosic ethanol industry. The transformation of Saccharomyces cerevisiae for xylose metabolism has become one of the research hotspot at present. In order to study the influence of CIT2 gene on xylose utilization of Saccharomyces cerevisiae strain C5D-P-M for glucose and xylose co-fermentation, the CIT2 gene disruption cassette produced by overlapping PCR technology, transformed into C5D-P-M for knockout of CIT2 by homologous recombination and mutant strain C5D-P-M-CIT2Δ was gained finally. In contrast with C5D-P-M, the growth rate, glucose utilization, xylose utilization, and ethanol production of C5D-P-M-CIT2Δ had improved to some extent. The result suggested CIT2 gene is one factor that can influence the xylose utilization and its knockout can improve xylose utilization of Saccharomyces cerevisiae.

Key words: Saccharomyces cerevisiae, CIT2 gene, gene knockout, xylose metabolism