关键词: ABP9基因, BAC文库, 染色体定位, 荧光原位杂交, 分子育种

Abstract: In order to obtain the accurate genomic sequence and its flanking genomic sequences of maize transcription factor ABP9, PCR method was used to screen the BAC library of maize inbred line Qi319 for identification of single BACs which contain ABP9. As a result, four positive single BACs were obtained from two randomly selected among 19 positive primary pools, namely BAC-103, BAC-159. By sequencing and sequence alignment of single clone BAC-103-C3 with Mo17 genome, ABP9 was mapped on chromosome 3 of Mo17. This result was confirmed by fluorescence in situ hybridization using green fluorescent-labeled ABP9 ORF as the probe with root tip chromosomes drops of germinating seeds of maize inbred lines Qi319 and Mo17. The knowledge of accurate genomic sequence and chromosomal localization of ABP9 will facilitate the development of molecular markers of this gene for use in molecular breeding.

Key words: ABP9 gene, BAC library, chromosome localization, fluorescence in situ hybridization, molecular breeding