关键词: 基因工程抗体, 分子模拟, 基因突变, 亲和力成熟

Abstract: Performance improvements of recombinant antibodies can be achieved by gene sequence analysis and modification. The methods for enhancing the affinity of recombinant antibodies through gene mutation were summarized, with special focus on the machanism analysis of small organic molecules-antibody interactions， antibody sequence databases and computer simulation based technologies，and a trending technology of molecular modeling and docking technology.

Key words: genetically engineered antibody, molecular modeling, gene mutation, affinity maturation