生物技术进展 ›› 2023, Vol. 13 ›› Issue (4): 604-611.DOI: 10.19586/j.2095-2341.2023.0030

• 研究论文 • 上一篇    

真菌Stilbella sp. CGMCC 40422的萜类产物研究

宋开南(), 艾羽桐, 徐玉泉()   

  1. 中国农业科学院生物技术研究所,北京 100081
  • 收稿日期:2023-03-14 接受日期:2023-05-06 出版日期:2023-07-25 发布日期:2023-08-03
  • 通讯作者: 徐玉泉
  • 作者简介:宋开南 E-mail: S15850653283@126.com
  • 基金资助:
    国家自然科学基金项目(22277137)

Study of the Terpenoids from Stilbella sp. CGMCC 40422

Kainan SONG(), Yutong AI, Yuquan XU()   

  1. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2023-03-14 Accepted:2023-05-06 Online:2023-07-25 Published:2023-08-03
  • Contact: Yuquan XU

摘要:

采用多种硅胶柱色谱、OSD柱色谱与半制备柱高效液相色谱对真菌Stilbella sp. CGMCC 40422大米培养基的发酵产物进行分离提纯,并通过质谱、核磁共振波谱解析等方法对得到的化合物纯品进行结构鉴定。结果显示,从该菌株中分离得到2个原萜烷型四环三萜、1个倍半萜与5个杂萜类化合物,分别为烟曲霉酸、helvolinic acid、tricho-acorenol、ascofuranone、ilicicolin C、LL-Z1272?、deacetylchloronectrin和ascochlorin N-acetylglucosamine。其中烟曲霉酸的含量最高,随后采用高效液相色谱法测定不同发酵时间发酵物中主产物烟曲霉酸的产量,烟曲霉酸含量在培养15 d后达最大值3 g·kg-1,说明Stilbella sp. CGMCC 40422具有强大的萜类产物生产能力,研究结果为进一步开发萜类化合物生产的底盘细胞奠定了基础。

关键词: Stilbella sp.CGMCC40422, 萜类化合物, 烟曲霉酸, 底盘细胞

Abstract:

The compounds from Stilbella sp. CGMCC 40422 were isolated by silica gel column, ODS column and semi-preparative high performance liquid chromatography. And their structures were elucidated by nuclear magnetic resonance (NMR) and mass spectrum (MS) spectral analysis. The results showed that two prototerpene-type tetracyclic triterpenoids, one sesquiterpenoid and five meroterpenoids were isolated from the rice fermentation products of the fungus, and their structures were identified as helvolic acid, helvolinic acid, tricho-acorenol, ascofuranone, ilicicolin C, LL-Z1272?, deacetylchloronectrin ascochlorin and N-acetylglucosamine. The content of helvolic acid under different fermentation time was tested by HPLC chromatography. The yeild of helvolic acid reached a maximum of 3 g·kg-1 after 15 days of fermentation, showing that Stilbella sp. CGMCC 40422 have strong production capacity of terpenoids. The results could lay the foundation for further development of the chassis cells of terpenoid cell factories.

Key words: Stilbella sp. CGMCC 40422, terpenoids, helvolic acid, chassis cells

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